[Determination of N,N'-ethylenediamine disuccinic acid in bioconversion reaction solution using high performance liquid chromatography].
Se Pu
; 37(10): 1118-1123, 2019 Oct 08.
Article
em Zh
| MEDLINE
| ID: mdl-31642292
ABSTRACT
A method was developed for the determination of N,N'-ethylenediamine disuccinic acid (EDDS) in bioconversion samples by high performance liquid chromatography (HPLC). An InertSustain AQ-C18 column (250 mm×4.6 mm, 5 µm) was used in the analysis. The mobile phase was 25% (v/v) methanol with 1.0 g/L Cu(CO2CH3)2·H2O, 2.0 g/L tetrabutylammonium hydroxide, and the pH was adjusted to 2.80 with phosphoric acid. The flow rate of the mobile phase was 1.0 mL/min, and the column temperature was set at 30â. The detection wavelength was 254 nm. EDDS, fumaric acid, citric acid, malic acid and ethylenediaminetetraacetic acid (EDTA) were separated from one another within 8 min. EDDS showed good linearity in the range of 0.06-0.6 g/L. About 0.25 g/L EDDS was detected in the biosynthesis reaction solution catalyzed by recombinant EDDS-lyase, while 36.56 g/L malic acid was formed as the by-product from fumaric acid. The catalytic activity of the enzyme was confirmed in the hydrolysis of 10 g/L EDDS, which produced 3.05 g/L malic acid in 3 h. This analytical method is simple, rapid, sensitive, reliable, and suitable for the analysis in the research of EDDS bioconversion process.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Succinatos
/
Cromatografia Líquida de Alta Pressão
/
Etilenodiaminas
Idioma:
Zh
Revista:
Se Pu
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China