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Expression-Based Cell Lineage Analysis in Drosophila Through a Course-Based Research Experience for Early Undergraduates.
Olson, John M; Evans, Cory J; Ngo, Kathy T; Kim, Hee Jong; Nguyen, Joseph Duy; Gurley, Kayla G H; Ta, Truc; Patel, Vijay; Han, Lisa; Truong-N, Khoa T; Liang, Letty; Chu, Maggie K; Lam, Hiu; Ahn, Hannah G; Banerjee, Abhik Kumar; Choi, In Young; Kelley, Ross G; Moridzadeh, Naseem; Khan, Awais M; Khan, Omair; Lee, Szuyao; Johnson, Elizabeth B; Tigranyan, Annie; Wang, Jay; Gandhi, Anand D; Padhiar, Manish M; Calvopina, Joseph Hargan; Sumra, Kirandeep; Ou, Kristy; Wu, Jessie C; Dickan, Joseph N; Ahmadi, Sabrena M; Allen, Donald N; Mai, Van Thanh; Ansari, Saif; Yeh, George; Yoon, Earl; Gon, Kimberly; Yu, John Y; He, Johnny; Zaretsky, Jesse M; Lee, Noemi E; Kuoy, Edward; Patananan, Alexander N; Sitz, Daniel; Tran, PhuongThao; Do, Minh-Tu; Akhave, Samira J; Alvarez, Silverio D; Asem, Bobby.
Afiliação
  • Olson JM; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Evans CJ; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095 Cory.Evans@lmu.edu banerjee@mbi.ucla.edu.
  • Ngo KT; Biomedical Research Minor, University of California, Los Angeles.
  • Kim HJ; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Nguyen JD; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Gurley KGH; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Ta T; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Patel V; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Han L; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Truong-N KT; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Liang L; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Chu MK; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Lam H; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Ahn HG; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Banerjee AK; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Choi IY; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Kelley RG; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Moridzadeh N; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Khan AM; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Khan O; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Lee S; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Johnson EB; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Tigranyan A; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Wang J; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Gandhi AD; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Padhiar MM; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Calvopina JH; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Sumra K; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Ou K; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Wu JC; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Dickan JN; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Ahmadi SM; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Allen DN; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Mai VT; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Ansari S; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Yeh G; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Yoon E; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Gon K; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Yu JY; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • He J; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Zaretsky JM; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Lee NE; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Kuoy E; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Patananan AN; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Sitz D; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Tran P; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Do MT; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Akhave SJ; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Alvarez SD; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
  • Asem B; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
G3 (Bethesda) ; 9(11): 3791-3800, 2019 11 05.
Article em En | MEDLINE | ID: mdl-31690598
ABSTRACT
A variety of genetic techniques have been devised to determine cell lineage relationships during tissue development. Some of these systems monitor cell lineages spatially and/or temporally without regard to gene expression by the cells, whereas others correlate gene expression with the lineage under study. The GAL4 Technique for Real-time and Clonal Expression (G-TRACE) system allows for rapid, fluorescent protein-based visualization of both current and past GAL4 expression patterns and is therefore amenable to genome-wide expression-based lineage screens. Here we describe the results from such a screen, performed by undergraduate students of the University of California, Los Angeles (UCLA) Undergraduate Research Consortium for Functional Genomics (URCFG) and high school summer scholars as part of a discovery-based education program. The results of the screen, which reveal novel expression-based lineage patterns within the brain, the imaginal disc epithelia, and the hematopoietic lymph gland, have been compiled into the G-TRACE Expression Database (GED), an online resource for use by the Drosophila research community. The impact of this discovery-based research experience on student learning gains was assessed independently and shown to be greater than that of similar programs conducted elsewhere. Furthermore, students participating in the URCFG showed considerably higher STEM retention rates than UCLA STEM students that did not participate in the URCFG, as well as STEM students nationwide.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linhagem da Célula / Drosophila Limite: Animals Idioma: En Revista: G3 (Bethesda) Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linhagem da Célula / Drosophila Limite: Animals Idioma: En Revista: G3 (Bethesda) Ano de publicação: 2019 Tipo de documento: Article