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A generic cell surface ligand system for studying cell-cell recognition.
Denham, Eleanor M; Barton, Michael I; Black, Susannah M; Bridge, Marcus J; de Wet, Ben; Paterson, Rachel L; van der Merwe, P Anton; Goyette, Jesse.
Afiliação
  • Denham EM; Sir William Dunn School of Pathology, University of Oxford, Oxford, Oxfordshire, United Kingdom.
  • Barton MI; Sir William Dunn School of Pathology, University of Oxford, Oxford, Oxfordshire, United Kingdom.
  • Black SM; Sir William Dunn School of Pathology, University of Oxford, Oxford, Oxfordshire, United Kingdom.
  • Bridge MJ; Sir William Dunn School of Pathology, University of Oxford, Oxford, Oxfordshire, United Kingdom.
  • de Wet B; Sir William Dunn School of Pathology, University of Oxford, Oxford, Oxfordshire, United Kingdom.
  • Paterson RL; Sir William Dunn School of Pathology, University of Oxford, Oxford, Oxfordshire, United Kingdom.
  • van der Merwe PA; Sir William Dunn School of Pathology, University of Oxford, Oxford, Oxfordshire, United Kingdom.
  • Goyette J; Sir William Dunn School of Pathology, University of Oxford, Oxford, Oxfordshire, United Kingdom.
PLoS Biol ; 17(12): e3000549, 2019 12.
Article em En | MEDLINE | ID: mdl-31815943
Dose-response experiments are a mainstay of receptor biology studies and can reveal valuable insights into receptor function. Such studies of receptors that bind cell surface ligands are currently limited by the difficulty in manipulating the surface density of ligands at a cell-cell interface. Here, we describe a generic cell surface ligand system that allows precise manipulation of cell surface ligand densities over several orders of magnitude. These densities are robustly quantifiable, a major advance over previous studies. We validate the system for a range of immunoreceptors, including the T-cell receptor (TCR), and show that this generic ligand stimulates via the TCR at a similar surface density as its native ligand. We also extend our work to the activation of chimeric antigen receptors. This novel system allows the effect of varying the surface density, valency, dimensions, and affinity of the ligand to be investigated. It can be readily broadened to other receptor-cell surface ligand interactions and will facilitate investigation into the activation of, and signal integration between, cell surface receptors.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bioensaio / Comunicação Celular / Antígenos de Superfície Limite: Animals / Humans Idioma: En Revista: PLoS Biol Assunto da revista: BIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bioensaio / Comunicação Celular / Antígenos de Superfície Limite: Animals / Humans Idioma: En Revista: PLoS Biol Assunto da revista: BIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Reino Unido