Assessment of clonality in gastrointestinal cancer by DNA fingerprinting.
J Clin Invest
; 82(5): 1532-7, 1988 Nov.
Article
em En
| MEDLINE
| ID: mdl-3183051
ABSTRACT
DNA fingerprinting with three different probes (33.15, 33.6, and alpha-globin 3'HVR) was investigated as a method for the determination of clonality in gastrointestinal tumors. In 29/44 carcinomas the tumor DNA showed clonal somatic mutations that were not seen in the corresponding peripheral blood and normal mucosa samples. The changes consisted of either novel fingerprint bands, losses of bands, or both. The probe 33.15 yielded the highest rate of abnormal DNA fingerprints (21/44 carcinomas). Sequential use of the probes increased the number of cases where clonal fingerprint markers could be detected. One out of five colorectal adenomas also showed a clonal loss of a fingerprint band. In two cases of gastric cancer, DNA from the metastatic tumor had a different DNA fingerprint from that found in the primary carcinoma. DNA fingerprinting offers a novel approach to determining clonality in tumors and may prove useful for the study of tumor progression.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mapeamento de Nucleotídeos
/
Clonagem Molecular
/
Neoplasias Gastrointestinais
Limite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Clin Invest
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
Reino Unido