Continuous Surveillance of Bioaerosols On-Site Using an Automated Bioaerosol-Monitoring System.
ACS Sens
; 5(2): 395-403, 2020 02 28.
Article
em En
| MEDLINE
| ID: mdl-31913022
ABSTRACT
Real-time on-site monitoring of bioaerosols in an air environment is important for preventing various adverse health effects including respiratory diseases and allergies caused by bioaerosols. Here, we report the development of an on-site automated bioaerosol-monitoring system (ABMS) using integrated units including a wet-cyclone bioaerosol sampler, a thermal-lysis unit for extracting adenosine triphosphate (ATP), an ATP-detection unit based on the immobilization of luciferase/luciferin for bioluminescence reactions, and a photomultiplier tube-based detector. The performance of the bioaerosol detection system was verified using Escherichia coli (E. coli) as a model source. Each unit was optimized to process â¼9.6 × 105 times the concentrated ratio of collected bioaerosol samples, using a 3 min lysis time to extract ATP, and has a detection limit of â¼375 colony-forming units (CFUs)/mL with more than 30 days of stability for the immobilized-luciferase/luciferin detection unit supported by a glass-fiber conjugation pad. After the integration of all units, the ABMS achieved E. coli bioaerosol monitoring with continuous detection at 5 min intervals and a minimum detection limit of â¼130 CFU/mair3. Furthermore, the rapid responsivity and stable operation performance of the ABMS under test-bed conditions and during a field test demonstrated that the ABMS is capable of continuously monitoring bioaerosols in real-time with high sensitivity. The monitoring system developed here with immobilization strategies for bioluminescence reactions triggered by ATP extracted from collected bioaerosol samples using a simple heat-lysis method may help establish sustainable platforms to obtain stable signals for the real-time detection of bioaerosols on-site.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Monitoramento Ambiental
/
Aerossóis
Tipo de estudo:
Screening_studies
Limite:
Humans
Idioma:
En
Revista:
ACS Sens
Ano de publicação:
2020
Tipo de documento:
Article