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piR-823 demonstrates tumor oncogenic activity in esophageal squamous cell carcinoma through DNA methylation induction via DNA methyltransferase 3B.
Su, Jing-Fen; Zhao, Fang; Gao, Zhao-Wei; Hou, Yong-Jie; Li, Yuan-Yuan; Duan, Li-Juan; Lun, Shu-Min; Yang, Hai-Jun; Li, Jun-Kuo; Dai, Ning-Tao; Shen, Fang-Fang; Zhou, Fu-You.
Afiliação
  • Su JF; Anyang Key Laboratory for Esophageal Cancer Research, Anyang Cancer Hospital, the Forth Affiliated Hospital of Henan University of Science and Technology, Anyang, Henan, People's Republic of China.
  • Zhao F; Anyang Key Laboratory for Esophageal Cancer Research, Anyang Cancer Hospital, the Forth Affiliated Hospital of Henan University of Science and Technology, Anyang, Henan, People's Republic of China.
  • Gao ZW; Anyang Key Laboratory for Esophageal Cancer Research, Anyang Cancer Hospital, the Forth Affiliated Hospital of Henan University of Science and Technology, Anyang, Henan, People's Republic of China.
  • Hou YJ; Anyang Key Laboratory for Esophageal Cancer Research, Anyang Cancer Hospital, the Forth Affiliated Hospital of Henan University of Science and Technology, Anyang, Henan, People's Republic of China.
  • Li YY; Biotecan company, Shanghai, People's Republic of China.
  • Duan LJ; Anyang Key Laboratory for Esophageal Cancer Research, Anyang Cancer Hospital, the Forth Affiliated Hospital of Henan University of Science and Technology, Anyang, Henan, People's Republic of China.
  • Lun SM; Anyang Key Laboratory for Esophageal Cancer Research, Anyang Cancer Hospital, the Forth Affiliated Hospital of Henan University of Science and Technology, Anyang, Henan, People's Republic of China.
  • Yang HJ; Anyang Key Laboratory for Esophageal Cancer Research, Anyang Cancer Hospital, the Forth Affiliated Hospital of Henan University of Science and Technology, Anyang, Henan, People's Republic of China.
  • Li JK; Anyang Key Laboratory for Esophageal Cancer Research, Anyang Cancer Hospital, the Forth Affiliated Hospital of Henan University of Science and Technology, Anyang, Henan, People's Republic of China.
  • Dai NT; Anyang Key Laboratory for Esophageal Cancer Research, Anyang Cancer Hospital, the Forth Affiliated Hospital of Henan University of Science and Technology, Anyang, Henan, People's Republic of China.
  • Shen FF; Key Laboratory for Tumor Translational Medicine, The Third Affiliated Hospital, Xinxiang Medical University, Xinxiang, Henan, People's Republic of China.
  • Zhou FY; Anyang Key Laboratory for Esophageal Cancer Research, Anyang Cancer Hospital, the Forth Affiliated Hospital of Henan University of Science and Technology, Anyang, Henan, People's Republic of China. Electronic address: ayzhoufuyou@163.com.
Pathol Res Pract ; 216(4): 152848, 2020 Apr.
Article em En | MEDLINE | ID: mdl-32051106
Piwi-interacting RNAs (piRNAs) dysregulation occurs frequently in extensive cancers. However, there was no report about piRNA expression in esophageal cancer (EC). In this study, the expression levels of piR-823 and DNMT1, DNMT3A, DNMT3B were detected in 54 pairs of ESCC tissues and adjacent normal tissues using the quantitative real-time polymerase chain reaction method. Pearson's chi-squared test and receiver operating characteristic curves were established to evaluate the diagnostic and prognostic value of piR-823 in ESCC. Spearman's correlation analysis was used to evaluate the association between piR-823 and DNMTs. We found that piR-823 was significantly upregulated in ESCC tissues compared with matched normal tissues (P = 0.0213), the level of piR-823 was significantly associated with lymph node metastasis (P = 0.042). The ROC curve analysis of piR-823 expression level yielded an area under the ROC curve value of 0.713 (P = 0.0001). DNMT3B was upregulated in ESCC tissues compared with matched normal tissues (P = 0.0286). There was an obvious positive correlation between piR-823 and DNMT3B expression (r = 0.6420, P < 0.0001). In conclusion, for the first time, we provided evidence about piRNA expression in EC. piRNA-823 and DNMT3B were both upregulated in ESCC and positively correlated with each other, suggesting the tumor oncogenic role of piR-823 in ESCC to epigenetically induce aberrant DNA methylation through DNMT3B. In addition, piRNA-823 showed high specificity in detecting ESCC and higher piRNA-823 level indicated higher risk of lymph node metastasis, suggesting its diagnostic and prognostic biomarker potential.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Esofágicas / Biomarcadores Tumorais / Regulação Neoplásica da Expressão Gênica / RNA Interferente Pequeno / DNA (Citosina-5-)-Metiltransferases / Carcinoma de Células Escamosas do Esôfago Tipo de estudo: Prognostic_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Pathol Res Pract Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Esofágicas / Biomarcadores Tumorais / Regulação Neoplásica da Expressão Gênica / RNA Interferente Pequeno / DNA (Citosina-5-)-Metiltransferases / Carcinoma de Células Escamosas do Esôfago Tipo de estudo: Prognostic_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Pathol Res Pract Ano de publicação: 2020 Tipo de documento: Article