A multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants.
Arch Virol
; 165(5): 1211-1214, 2020 May.
Article
em En
| MEDLINE
| ID: mdl-32170392
ABSTRACT
Papaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
/
Totiviridae
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Carica
/
Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Arch Virol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Brasil