Vectors for efficient expression in mammalian fibroblastoid, myeloid and lymphoid cells via transfection or infection.
Gene
; 68(2): 213-9, 1988 Sep 07.
Article
em En
| MEDLINE
| ID: mdl-3220255
ABSTRACT
We have constructed two related types of multi-cloning mammalian expression vectors. The first, pMPSVEH/HE, carries the promoter of the myeloproliferative sarcoma virus (MPSV). This promoter was found to be stronger than both the SV40 early and the trans-activated human immunodeficiency virus promoters in many cell lines including human and rodent fibroblastoid, lymphoid or myeloid cells. The other, pBEH/HE, carries the simian virus 40 (SV40) early promoter and origin of replication. This offers the possibility of encapsidation in SV40 pseudovirions and subsequent gene transfer into, e.g., hemopoietic cells, via infection. The usefulness of the expression systems was tested with a number of genes and cell lines.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transfecção
/
Transformação Celular Viral
/
Clonagem Molecular
/
Vetores Genéticos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Gene
Ano de publicação:
1988
Tipo de documento:
Article