A nanoplatform based on metal-organic frameworks and coupled exonuclease reaction for the fluorimetric determination of T4 polynucleotide kinase activity and inhibition.

Chai, Yuying; Cheng, Xia; Xu, Guanhong; Wei, Fangdi; Bao, Jian; Mei, Jie; Ren, Dandan; Hu, Qin; Cen, Yao

Chai, Yuying; Cheng, Xia; Xu, Guanhong; Wei, Fangdi; Bao, Jian; Mei, Jie; Ren, Dandan; Hu, Qin; Cen, Yao.

Afiliação

Chai Y; School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, People's Republic of China.
Cheng X; School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, People's Republic of China.
Xu G; School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, People's Republic of China.
Wei F; School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, People's Republic of China.
Bao J; School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, People's Republic of China.
Mei J; School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, People's Republic of China.
Ren D; School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, People's Republic of China.
Hu Q; School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, People's Republic of China. huqin@njmu.edu.cn.
Cen Y; School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, People's Republic of China. yaocen@njmu.edu.cn.

Mikrochim Acta ; 187(4): 243, 2020 03 23.

Article em En | MEDLINE | ID: mdl-32206934

Assuntos

Bacteriófago T4/enzimologia; Fluorometria/métodos; Estruturas Metalorgânicas/química; Nanotecnologia/métodos; Polinucleotídeo 5'-Hidroxiquinase/metabolismo; DNA de Cadeia Simples/química; Inibidores Enzimáticos/análise; Exonucleases/metabolismo; Fluorescência; Limite de Detecção; Polinucleotídeo 5'-Hidroxiquinase/antagonistas & inibidores

Palavras-chave

Cell extracts; Coupled enzyme reaction; Fe-MIL-88; Fluorescence assay; Inhibitor screening; Lambda exonuclease; MOFs; Preferential binding property; Static and dynamic quenching; T4 PNK

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polinucleotídeo 5'-Hidroxiquinase / Bacteriófago T4 / Nanotecnologia / Fluorometria / Estruturas Metalorgânicas Idioma: En Revista: Mikrochim Acta Ano de publicação: 2020 Tipo de documento: Article

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