Hydrated electrons induce the formation of interstrand cross-links in DNA modified by cisplatin adducts.
J Radiat Res
; 61(3): 343-351, 2020 May 22.
Article
em En
| MEDLINE
| ID: mdl-32211848
ABSTRACT
Double-stranded oligonucleotides containing cisplatin adducts, with and without a mismatched region, were exposed to hydrated electrons generated by gamma-rays. Gel electrophoresis analysis demonstrates the formation of cisplatin-interstrand crosslinks from the cisplatin-intrastrand species. The rate constant per base for the reaction between hydrated electrons and the double-stranded oligonucleotides with and without cisplatin containing a mismatched region was determined by pulse radiolysis to be 7 × 109 and 2 × 109 M-1 s-1, respectively. These results provide a better understanding of the radiosensitizing effect of cisplatin adducts in hypoxic tumors and of the formation of interstrand crosslinks, which are difficult for cells to repair.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
DNA
/
Cisplatino
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Adutos de DNA
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Reagentes de Ligações Cruzadas
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Elétrons
Limite:
Humans
Idioma:
En
Revista:
J Radiat Res
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Canadá