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Pinpoint analysis of a protein in slow exchange using F1F2-selective ZZ-exchange spectroscopy: assignment and kinetic analysis.
Nishizawa, Mayu; Walinda, Erik; Morimoto, Daichi; Sugase, Kenji.
Afiliação
  • Nishizawa M; Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku Katsura, Nishikyo-Ku, Kyoto, 615-8510, Japan.
  • Walinda E; Department of Molecular and Cellular Physiology, Graduate School of Medicine, Kyoto University, Sakyo-ku Yoshida Konoe-cho, Kyoto, 606-8501, Japan.
  • Morimoto D; Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku Katsura, Nishikyo-Ku, Kyoto, 615-8510, Japan.
  • Sugase K; Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku Katsura, Nishikyo-Ku, Kyoto, 615-8510, Japan. sugase@moleng.kyoto-u.ac.jp.
J Biomol NMR ; 74(4-5): 205-211, 2020 May.
Article em En | MEDLINE | ID: mdl-32236785
ABSTRACT
ZZ-exchange spectroscopy is widely used to study slow exchange processes in biomolecules, especially determination of exchange rates and assignment of minor peaks. However, if the exchange cross peaks overlap or the populations are skewed, kinetic analysis is hindered. In order to analyze slow exchange protein dynamics under such conditions, here we have developed a new method by combining ZZ-exchange and F1F2-selective NMR spectroscopy. We demonstrate the utility of this method by examining the monomer-dimer transition of the ubiquitin-associated domain of p62, successfully assigning the minor (monomeric) peaks and obtaining the exchange rates, which cannot be achieved by ZZ-exchange alone.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ressonância Magnética Nuclear Biomolecular / Proteína Sequestossoma-1 Limite: Humans Idioma: En Revista: J Biomol NMR Assunto da revista: BIOLOGIA MOLECULAR / DIAGNOSTICO POR IMAGEM / MEDICINA NUCLEAR Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ressonância Magnética Nuclear Biomolecular / Proteína Sequestossoma-1 Limite: Humans Idioma: En Revista: J Biomol NMR Assunto da revista: BIOLOGIA MOLECULAR / DIAGNOSTICO POR IMAGEM / MEDICINA NUCLEAR Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Japão