Antibody responses in human gnathostomiasis.
Southeast Asian J Trop Med Public Health
; 19(2): 219-24, 1988 Jun.
Article
em En
| MEDLINE
| ID: mdl-3227402
ABSTRACT
Sera from 4 patients with parasitologically confirmed gnathostomiasis and from 18 healthy individuals were studied by SDS-PAGE and Western blot analysis using radioiodinated protein A to detect antibody responses against crude aqueous somatic extract of advanced third stage larvae of Gnathostoma spinigerum (L3G). It was found that the L3G extract was highly complex, comprising of more than 40 polypeptides among which more than 20 components were antigenic in human. The relative M.W. of the proteins ranged from 13 kd to 150 kd with the major antigenic bands at 150, 135, 120, 94, 84, 82, 72, 55, 54, 49, 43, 38, 35, 32 and 28 kd. All 4 sera from gnathostomiasis patients gave almost an identical pattern of reactivities against the L3G antigens whereas sera from the normal individuals gave much lower reactivities against the L3G antigen of M.W. 38 kd and, in certain individuals, those of 49 and 43 kd. The present findings suggest that the serum antibody response against the parasite is specific and may be useful in a specific or a confirmed immunodiagnosis of human gnathostomiasis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anticorpos Anti-Helmínticos
/
Reações Antígeno-Anticorpo
/
Antígenos de Helmintos
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Infecções por Nematoides
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Southeast Asian J Trop Med Public Health
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
Tailândia