Retention reproducibility of basic drugs in high-performance liquid chromatography on a silica column with a methanol-ammonium nitrate eluent. Batch-to-batch reproducibility of the stationary phase.
J Chromatogr
; 455: 77-93, 1988 Nov 25.
Article
em En
| MEDLINE
| ID: mdl-3235625
ABSTRACT
The effect of changing the brand and batches of the silica stationary phase used with a methanol-aqueous ammonium nitrate eluent for the high-performance liquid chromatographic separation of basic drugs has been studied. Considerable care had to be taken to obtain a reproducible eluent and the effect of small changes in the concentration of the ammonia solution were examined closely. Large differences in both the capacity factor and relative capacity factors were found for separations on columns packed with four different brands of silica. Significant differences were also observed with columns containing eighteen different batches of Spherisorb S5W, which had been manufactured over a period of several years.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preparações Farmacêuticas
/
Cromatografia Líquida de Alta Pressão
Idioma:
En
Revista:
J Chromatogr
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
Reino Unido