Enzymatic initiation of DNA synthesis by yeast DNA polymerases.
Proc Natl Acad Sci U S A
; 74(5): 1937-41, 1977 May.
Article
em En
| MEDLINE
| ID: mdl-325562
ABSTRACT
Partially purified yeast RNA polymerases (RNA nucleotidyltransferases) initiate DNA synthesis by yeast DNA polymerase (DNA nucleotidyltransferase) I and to a lesser extent yeast DNA polymerase II in the replication of single-stranded DNA. The enzymatic initiation of DNA synthesis on phage fd DNA template occurs with dNTPs alone and is further stimulated by the presence of rNTPs in DNA polymerase I reactions. The presence of rNTPs has no effect on the RNA polymerase initiation of the DNA polymerase II reaction. RNA polymerases I and III are more efficient in initiation of DNA synthesis than RNA polymerase II. Analyses of the products of fd DNA replication show noncovalent linkage between the newly synthesized DNA and the template DNA, and covalent linkage between the newly synthesized RNA and DNA.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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RNA Polimerases Dirigidas por DNA
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DNA
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DNA Polimerase Dirigida por DNA
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
1977
Tipo de documento:
Article