Collaborative study: Quantification of total folate in food using an efficient single-enzyme extraction combined with LC-MS/MS.
Food Chem
; 333: 127447, 2020 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-32688304
ABSTRACT
Quantification of the specific folate vitamers to estimate total folate in foods is not standardized. A collaborative study, including eight European laboratories, was conducted in order to determine the repeatability and reproducibility of the method for folate quantification in foods using the plant-origin γ-glutamyl hydrolase as part of the extraction procedure. The seven food samples analyzed represent the food groups; fruits, vegetables, dairy products, legumes, offal, fish, and fortified infant formula. The homogenization step was included, and six folate vitamers were analyzed using LC-MS/MS. Total folate content, expressed as folic acid equivalent, was 17-490 µg/100 g in all samples. Horwitz ratio values were within the acceptable range (0.60-1.94), except for fish. The results for fortified infant formula, a certified reference material (NIST 1869), confirmed the trueness of the method. The collaborative study is part of a standardization project within the Nordic Committee on Food Analysis (NMKL).
Palavras-chave
10-Formylfolic acid (PubChem CID: 135405023); 5,10-Methenyltetrahydrofolate (PubChem CID: 135398657); 5-Formyltetrahydrofolate (PubChem CID: 135402009); 5-Methyltetrahydrofolate (PubChem CID: 135415868); Deconjugase; Folic acid (PubChem CID: 135398658); Homogenization; Inter-laboratory study; Plant enzyme; Tetrahydrofolate (PubChem CID: 135444742); Tetrahydrofolates
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas em Tandem
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Ácido Fólico
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Análise de Alimentos
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Fracionamento Químico
Limite:
Animals
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Humans
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Infant
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2020
Tipo de documento:
Article