Homology analysis of 35 ß-glucosidases in Oenococcus oeni and biochemical characterization of a novel ß-glucosidase BGL0224.
Food Chem
; 334: 127593, 2021 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-32711276
ABSTRACT
ß-Glucosidases play an important role in food industry. Oenococcus oeni are typical lactic acid bacteria that initiate malolactic fermentation of wines. 35 ß-glucosidases from O. oeni were selected and their conserved domains and evolutionary relationships were further explored in this study. The homology analysis results indicated that 35 ß-glucosidases were basically derived from GH1 and GH3 family. A novel ß-glucosidase was successfully expressed and characterized. The recombinant protein, referred to as BGL0224, consisted of a total 480 amino acids with an apparent molecular weight of 55.15 kDa and was classified as GH1 family. It achieved the highest activity at pH 5.0 and 50 °C. The activity and stability were significantly increased when 12% ethanol was supplemented to the enzyme. Using p-NPG as substrate, the Km, Vmax and Kcat of BGL0224 were 0.34 mM, 382.81 U/mg and 351.88 s-1, respectively. In all, BGL0224 has good application prospects in food industry.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vinho
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Beta-Glucosidase
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Oenococcus
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China