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Genomic signals found using RNA sequencing show signatures of selection and subtle population differentiation in walleye (Sander vitreus) in a large freshwater ecosystem.
Thorstensen, Matt J; Jeffrey, Jennifer D; Treberg, Jason R; Watkinson, Douglas A; Enders, Eva C; Jeffries, Ken M.
Afiliação
  • Thorstensen MJ; Department of Biological Sciences University of Manitoba Winnipeg MB Canada.
  • Jeffrey JD; Department of Biological Sciences University of Manitoba Winnipeg MB Canada.
  • Treberg JR; Department of Biological Sciences University of Manitoba Winnipeg MB Canada.
  • Watkinson DA; Freshwater Institute, Fisheries and Oceans Canada Winnipeg MB Canada.
  • Enders EC; Freshwater Institute, Fisheries and Oceans Canada Winnipeg MB Canada.
  • Jeffries KM; Department of Biological Sciences University of Manitoba Winnipeg MB Canada.
Ecol Evol ; 10(14): 7173-7188, 2020 Jul.
Article em En | MEDLINE | ID: mdl-32760520
ABSTRACT
RNA sequencing is an effective approach for studying aquatic species yielding both physiological and genomic data. However, its population genetic applications are not well-characterized. We investigate this possible role for RNA sequencing for population genomics in Lake Winnipeg, Manitoba, Canada, walleye (Sander vitreus). Lake Winnipeg walleye represent the largest component of the second-largest freshwater fishery in Canada. In the present study, large female walleye were sampled via nonlethal gill biopsy over two years at three spawning sites representing a latitudinal gradient in the lake. Genetic variation from sequenced mRNA was analyzed for neutral and adaptive markers to investigate population structure and possible adaptive variation. We find low population divergence (F ST = 0.0095), possible northward gene flow, and outlier loci that vary latitudinally in transcripts associated with cell membrane proteins and cytoskeletal function. These results indicate that Lake Winnipeg walleye may be effectively managed as a single demographically connected metapopulation with contributing subpopulations and suggest genomic differences possibly underlying observed phenotypic differences. Despite its high cost relative to other genotyping methods, RNA sequencing data can yield physiological in addition to genetic information discussed here. We therefore argue that it is useful for addressing diverse molecular questions in the conservation of freshwater species.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Ecol Evol Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Ecol Evol Ano de publicação: 2020 Tipo de documento: Article