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mRNA adenosine methylase (MTA) deposits m6A on pri-miRNAs to modulate miRNA biogenesis in Arabidopsis thaliana.
Bhat, Susheel Sagar; Bielewicz, Dawid; Gulanicz, Tomasz; Bodi, Zsuzsanna; Yu, Xiang; Anderson, Stephen J; Szewc, Lukasz; Bajczyk, Mateusz; Dolata, Jakub; Grzelak, Natalia; Smolinski, Dariusz J; Gregory, Brian D; Fray, Rupert G; Jarmolowski, Artur; Szweykowska-Kulinska, Zofia.
Afiliação
  • Bhat SS; Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, Poland.
  • Bielewicz D; Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, Poland.
  • Gulanicz T; Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, Poland.
  • Bodi Z; Centre For Modern Interdisciplinary Technologies, Nicolaus Copernicus University, 87-100 Torun, Poland.
  • Yu X; School of Biosciences, Plant Science Division, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom.
  • Anderson SJ; Department of Biology, University of Pennsylvania, Philadelphia, PA 19104.
  • Szewc L; Department of Biology, University of Pennsylvania, Philadelphia, PA 19104.
  • Bajczyk M; Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, Poland.
  • Dolata J; Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, Poland.
  • Grzelak N; Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, Poland.
  • Smolinski DJ; Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, 61-614 Poznan, Poland.
  • Gregory BD; Centre For Modern Interdisciplinary Technologies, Nicolaus Copernicus University, 87-100 Torun, Poland.
  • Fray RG; Department of Cellular and Molecular Biology, Nicolaus Copernicus University, 87-100 Torun, Poland.
  • Jarmolowski A; Department of Biology, University of Pennsylvania, Philadelphia, PA 19104.
  • Szweykowska-Kulinska Z; School of Biosciences, Plant Science Division, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom.
Proc Natl Acad Sci U S A ; 117(35): 21785-21795, 2020 09 01.
Article em En | MEDLINE | ID: mdl-32817553
In Arabidopsis thaliana, the METTL3 homolog, mRNA adenosine methylase (MTA) introduces N6-methyladenosine (m6A) into various coding and noncoding RNAs of the plant transcriptome. Here, we show that an MTA-deficient mutant (mta) has decreased levels of microRNAs (miRNAs) but accumulates primary miRNA transcripts (pri-miRNAs). Moreover, pri-miRNAs are methylated by MTA, and RNA structure probing analysis reveals a decrease in secondary structure within stem-loop regions of these transcripts in mta mutant plants. We demonstrate interaction between MTA and both RNA Polymerase II and TOUGH (TGH), a plant protein needed for early steps of miRNA biogenesis. Both MTA and TGH are necessary for efficient colocalization of the Microprocessor components Dicer-like 1 (DCL1) and Hyponastic Leaves 1 (HYL1) with RNA Polymerase II. We propose that secondary structure of miRNA precursors induced by their MTA-dependent m6A methylation status, together with direct interactions between MTA and TGH, influence the recruitment of Microprocessor to plant pri-miRNAs. Therefore, the lack of MTA in mta mutant plants disturbs pri-miRNA processing and leads to the decrease in miRNA accumulation. Furthermore, our findings reveal that reduced miR393b levels likely contributes to the impaired auxin response phenotypes of mta mutant plants.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: MicroRNAs / Metiltransferases Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Polônia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: MicroRNAs / Metiltransferases Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Polônia