A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR.
Chem Commun (Camb)
; 56(78): 11665-11668, 2020 Oct 07.
Article
em En
| MEDLINE
| ID: mdl-33000772
ABSTRACT
The activity of membrane proteins and compounds that interact with the membrane is modulated by the surrounding lipid composition. However, there are no simple methods that determine the composition of these annular phospholipids in eukaryotic systems. Herein, we describe a simple methodology that enables the identification and quantification of the lipid composition around membrane-associated compounds using SMA-nanodiscs and routine 1H-31P NMR.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
/
Espectroscopia de Ressonância Magnética
/
Proteínas de Membrana
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
Chem Commun (Camb)
Assunto da revista:
QUIMICA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Reino Unido