LncRNA-P21 suppresses apoptosis of myocardial cells in rats with acute myocardial infarction via regulating Wnt/ß-catenin signaling pathway.
Eur Rev Med Pharmacol Sci
; 24(19): 10078-10085, 2020 10.
Article
em En
| MEDLINE
| ID: mdl-33090415
ABSTRACT
OBJECTIVE:
The aim of this study was to investigate the expression of long non-coding ribonucleic acid (lncRNA)-p21 in rats with acute myocardial infarction (AMI) and its influences on the viability and apoptosis of myocardial cells. MATERIALS ANDMETHODS:
Sprague-Dawley rats were utilized to establish the AMI model. Myocardial tissues were extracted, and myocardial cells were isolated. Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the expression of lncRNA-p21. Subsequently, myocardial cells of AMI rats were cultured and transfected with lncRNA-p21 or small interfering (si)-lncRNA-p21. 48 h later, cell proliferation was determined using Cell Counting Kit-8 (CCK-8). Caspase-3 kit was applied to examine the changes in Caspase-3 after myocardial cell transfection. Moreover, Western blotting assay was performed to measure the protein expressions of apoptosis-associated indexes [B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax)] and key members of the Wnt signaling pathway (Wnt-5a and ß-catenin).RESULTS:
The expression of lncRNA-p21 decreased significantly in myocardial tissues (p<0.01). However, the mRNA expression of inflammatory factors was significantly upregulated in AMI group compared with those in Sham group (p<0.01). Compared with Vehicle group, LncRNA-p21 group exhibited remarkably enhanced myocardial cell viability (p<0.05). However, Si-lncRNA-p21 group weakened myocardial cell viability (p<0.05). The expression of Caspase-3 in myocardial cells was reduced in LncRNA-p21 group (p<0.05), but remarkably rose in Si-lncRNA-p21 group (p<0.05) in comparison with Vehicle group. In addition, the protein expressions of Wnt-5a and ß-catenin in myocardial cells were significantly higher in LncRNA-p21 group (p<0.05), whereas were lower in Si-lncRNA-p21 group (p<0.05) than those in Vehicle group.CONCLUSIONS:
LncRNA-p21 was lowly expressed in myocardial tissues of AMI rats. Furthermore, it affected the proliferation, apoptosis and inflammation level of myocardial cells in AMI rats by activating the Wnt/ß-catenin signaling pathway.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Miócitos Cardíacos
/
Via de Sinalização Wnt
/
RNA Longo não Codificante
/
Infarto do Miocárdio
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Eur Rev Med Pharmacol Sci
Assunto da revista:
FARMACOLOGIA
/
TOXICOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China