Molecular Reorganization during the Formation of the Human Skin Barrier Studied In Situ.
J Invest Dermatol
; 141(5): 1243-1253.e6, 2021 05.
Article
em En
| MEDLINE
| ID: mdl-33098827
ABSTRACT
In vertebrates, skin upholds homeostasis by preventing body water loss. The skin's permeability barrier is located intercellularly in the stratum corneum and consists of stacked lipid lamellae composed of ceramides, cholesterol, and free fatty acids. We have combined cryo-electron microscopy with molecular dynamics modeling and electron microscopy simulation in our analysis of the lamellae's formation, a maturation process beginning in stratum granulosum and ending in stratum corneum. Previously, we have revealed the lipid lamellae's initial- and end-stage molecular organizations. In this study, we reveal two cryo-electron microscopy patterns representing intermediate stages in the lamellae's maturation process a single-band pattern with 2.0â2.5 nm periodicity and a two-band pattern with 5.5â6.0 nm periodicity, which may be derived from lamellar lipid structures with 4.0â5.0 nm and 5.5â6.0 nm periodicity, respectively. On the basis of the analysis of the data now available on the four maturation stages identified, we can present a tentative molecular model for the complete skin barrier formation process.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
Limite:
Adult
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
J Invest Dermatol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Suécia