Spatially resolved absolute quantitation in thin tissue by mass spectrometry.
Anal Bioanal Chem
; 413(10): 2619-2636, 2021 Apr.
Article
em En
| MEDLINE
| ID: mdl-33140126
ABSTRACT
Mass spectrometry (MS) has become the de facto tool for routine quantitative analysis of biomolecules. MS is increasingly being used to reveal the spatial distribution of proteins, metabolites, and pharmaceuticals in tissue and interest in this area has led to a number of novel spatially resolved MS technologies. Most spatially resolved MS measurements are qualitative in nature due to a myriad of potential biases, such as sample heterogeneity, sampling artifacts, and ionization effects. As applications of spatially resolved MS in the pharmacological and clinical fields increase, demand has become high for quantitative MS imaging and profiling data. As a result, several varied technologies now exist that provide differing levels of spatial and quantitative information. This review provides an overview of MS profiling and imaging technologies that have demonstrated quantitative analysis from tissue. Focus is given on the fundamental processes affecting quantitative analysis in an array of MS imaging and profiling technologies and methods to address these biases.Graphical abstract.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Técnicas Histológicas
Tipo de estudo:
Qualitative_research
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Anal Bioanal Chem
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos