Improved dual luciferase reporter (DLR) assay to determine the protein stability.

Sun, Yinglu; Zhao, Jinfeng; Patil, Suyash Bhimgonda; Fang, Jingjing; Liu, Jun; Li, Xueyong

Sun, Yinglu; Zhao, Jinfeng; Patil, Suyash Bhimgonda; Fang, Jingjing; Liu, Jun; Li, Xueyong.

Afiliação

Sun Y; National Key Facility for Crop Gene Resources and Genetic Improvement, ICS, CAAS, 12 Zhongguancun South Street, Haidian District, Beijing, 100081, China. Electronic address: 339058452@qq.com.
Zhao J; National Key Facility for Crop Gene Resources and Genetic Improvement, ICS, CAAS, 12 Zhongguancun South Street, Haidian District, Beijing, 100081, China. Electronic address: zhaojinfeng@caas.cn.
Patil SB; National Key Facility for Crop Gene Resources and Genetic Improvement, ICS, CAAS, 12 Zhongguancun South Street, Haidian District, Beijing, 100081, China. Electronic address: suyashpatil7@gmail.com.
Fang J; National Key Facility for Crop Gene Resources and Genetic Improvement, ICS, CAAS, 12 Zhongguancun South Street, Haidian District, Beijing, 100081, China. Electronic address: fangjingjing@caas.cn.
Liu J; National Key Facility for Crop Gene Resources and Genetic Improvement, ICS, CAAS, 12 Zhongguancun South Street, Haidian District, Beijing, 100081, China. Electronic address: liujun@caas.cn.
Li X; National Key Facility for Crop Gene Resources and Genetic Improvement, ICS, CAAS, 12 Zhongguancun South Street, Haidian District, Beijing, 100081, China. Electronic address: lixueyong@caas.cn.

Anal Biochem ; 612: 114021, 2021 01 01.

Article em En | MEDLINE | ID: mdl-33189704

Assuntos

Bioensaio/métodos; Genes Reporter; Luciferases/metabolismo; Estabilidade Proteica; Proteínas de Arabidopsis/genética; Proteínas de Arabidopsis/metabolismo; Vetores Genéticos; Compostos Heterocíclicos com 3 Anéis/farmacologia; Indicadores e Reagentes/química; Lactonas/farmacologia; Luciferases de Vaga-Lume/genética; Luciferases de Renilla/genética; Oryza/metabolismo; Reguladores de Crescimento de Plantas/genética; Reguladores de Crescimento de Plantas/metabolismo; Folhas de Planta/metabolismo; Protoplastos/metabolismo; Nicotiana/metabolismo

Palavras-chave

Dual luciferase reporter system; Protein abundance; Protein stability; Relative FF/REN ratio; StrigoQuant biosensor

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bioensaio / Genes Reporter / Estabilidade Proteica / Luciferases Idioma: En Revista: Anal Biochem Ano de publicação: 2021 Tipo de documento: Article

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