Pharmacognostic standardization and DNA fingerprinting of leaves of Datura stramonium, growing naturally in Asir region of Saudi Arabia.
Pak J Pharm Sci
; 33(3): 1155-1161, 2020 May.
Article
em En
| MEDLINE
| ID: mdl-33191242
ABSTRACT
Evaluation of the various pharmacognostic quality parameters and DNA fingerprint of Saudi Arabian medicinal plant namely Datura stramonium growing in Asir region was the objective of the study. The pharmacognostical parameters were done in terms of macroscopic characters, microscopic details, physico-chemical evaluations, phytochemical analysis, fluorescence analysis and DNA fingerprint by using standard techniques and random polymorphic DNA primer. The detailed microscopy of the leaf revealed the presence of pre-medullary phloem, xylem, endodermis, parenchymatous pericycle, lower epidermis and calcium oxalate crystals. There are various amounts of foreign material, ash values, moisture content and extractive values, found after estimations. Preliminary phytochemical screening exhibited the presence of alkaloids, flavonoids, glycosides, tannins and sterols in variable amounts. Random amplified polymorphic DNA (RAPD) showed there are a prominent scorbale DNA bands. These evaluations provided referential information for correct authentication and quality standardization of the important medicinal plant material. These information will also be supportive to differentiate Datura stramonium from the closely related other species.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Farmacognosia
/
Datura stramonium
/
Impressões Digitais de DNA
/
Folhas de Planta
/
DNA de Plantas
/
Compostos Fitoquímicos
País/Região como assunto:
Asia
Idioma:
En
Revista:
Pak J Pharm Sci
Assunto da revista:
FARMACIA
/
FARMACOLOGIA
/
QUIMICA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Arábia Saudita