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Tetrahedral DNA Nanoconjugates for Simultaneous Measurement of Telomerase Activity and miRNA.
Jiang, Chenyu; Meng, Fanyu; Mao, Dongsheng; Tang, Yuguo; Miao, Peng.
Afiliação
  • Jiang C; Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, P. R. China.
  • Meng F; Jinan Guokeyigong Science and Technology Development Co., Ltd., Jinan, 250103, P. R. China.
  • Mao D; Ji Hua Laboratory, Foshan, 528200, P. R. China.
  • Tang Y; School of Life Sciences, Shanghai University, Shanghai, 200444, P. R. China.
  • Miao P; Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, P. R. China.
Chembiochem ; 22(7): 1302-1306, 2021 04 06.
Article em En | MEDLINE | ID: mdl-33242223
In this study, a tetrahedral DNA nanostructure was first self-assembled; this was then conjugated with gold nanoparticles (AuNPs) and carbon nanodots (CDs). The fabricated nanocomposites allow simultaneous analysis of telomerase activity and miRNA with dual fluorescence channels. By further introducing an iRGD peptide sequence, the nanoconjuates can be conveniently transferred inside living cells for in situ imaging. The analytical performances and anti-jamming capabilities are excellent. Meanwhile, the materials are highly biocompatible for intracellular applications. Therefore, the proposed biosystem shows great promise as a powerful tool for quantitative analysis of the dual biomarkers. The strategy can also be further exploited as a versatile platform for in situ detection of many other targets for early disease diagnosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Fluorescência / DNA / Microscopia Confocal / Telomerase / MicroRNAs / Nanoconjugados Limite: Animals / Humans Idioma: En Revista: Chembiochem Assunto da revista: BIOQUIMICA Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Fluorescência / DNA / Microscopia Confocal / Telomerase / MicroRNAs / Nanoconjugados Limite: Animals / Humans Idioma: En Revista: Chembiochem Assunto da revista: BIOQUIMICA Ano de publicação: 2021 Tipo de documento: Article