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IGI-LuNER: single-well multiplexed RT-qPCR test for SARS-CoV-2.
Stahl, Elizabeth C; Tsuchida, Connor A; Hamilton, Jennifer R; Lin-Shiao, Enrique; McDevitt, Shana L; Moehle, Erica A; Witkowsky, Lea B; Tsui, C Kimberly; Pestal, Kathleen; Gildea, Holly K; McElroy, Matthew; Keller, Amanda; Sylvain, Iman; Williams, Clara; Hirsh, Ariana; Ciling, Alison; Ehrenberg, Alexander J; Urnov, Fyodor D; Ringeisen, Bradley R; Giannikopoulos, Petros; Doudna, Jennifer A.
Afiliação
  • Stahl EC; University of California, Berkeley, Berkeley, CA, USA.
  • Tsuchida CA; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.
  • Hamilton JR; University of California, Berkeley, Berkeley, CA, USA.
  • Lin-Shiao E; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.
  • McDevitt SL; University of California, Berkeley, Berkeley, CA, USA.
  • Moehle EA; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.
  • Witkowsky LB; University of California, Berkeley, Berkeley, CA, USA.
  • Tsui CK; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.
  • Pestal K; University of California, Berkeley, Berkeley, CA, USA.
  • Gildea HK; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.
  • McElroy M; University of California, Berkeley, Berkeley, CA, USA.
  • Keller A; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.
  • Sylvain I; University of California, Berkeley, Berkeley, CA, USA.
  • Williams C; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.
  • Hirsh A; University of California, Berkeley, Berkeley, CA, USA.
  • Ciling A; University of California, Berkeley, Berkeley, CA, USA.
  • Ehrenberg AJ; University of California, Berkeley, Berkeley, CA, USA.
  • Urnov FD; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.
  • Ringeisen BR; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.
  • Giannikopoulos P; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.
  • Doudna JA; University of California, Berkeley, Berkeley, CA, USA.
medRxiv ; 2020 Dec 11.
Article em En | MEDLINE | ID: mdl-33330883
ABSTRACT
Commonly used RT-qPCR-based SARS-CoV-2 diagnostics require 2-3 separate reactions or rely on detection of a single viral target, adding time and cost or risk of false-negative results. Currently, no test combines detection of widely used SARS-CoV-2 E- and N-gene targets and a sample control in a single, multiplexed reaction. We developed the IGI-LuNER RT-qPCR assay using the Luna Probe Universal One-Step RT-qPCR master mix with publicly available primers and probes to detect SARS-CoV-2 N gene, E gene, and human RNase P (NER). This combined, cost-effective test can be performed in 384-well plates with detection sensitivity suitable for clinical reporting, and will aid in future sample pooling efforts, thus improving throughput of SARS-CoV-2 detection.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: MedRxiv Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: MedRxiv Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Estados Unidos