Rapid and efficient cataract gene evaluation in F0 zebrafish using CRISPR-Cas9 ribonucleoprotein complexes.

Zhao, Duran; Jones, Johanna L; Gasperini, Robert J; Charlesworth, Jac C; Liu, Guei-Sheung; Burdon, Kathryn P

Zhao, Duran; Jones, Johanna L; Gasperini, Robert J; Charlesworth, Jac C; Liu, Guei-Sheung; Burdon, Kathryn P.

Afiliação

Zhao D; Menzies Institute for Medical Research, University of Tasmania, Hobart, Tasmania, Australia.
Jones JL; Menzies Institute for Medical Research, University of Tasmania, Hobart, Tasmania, Australia.
Gasperini RJ; School of Medicine, University of Tasmania, Hobart, Tasmania, Australia.
Charlesworth JC; Menzies Institute for Medical Research, University of Tasmania, Hobart, Tasmania, Australia.
Liu GS; Menzies Institute for Medical Research, University of Tasmania, Hobart, Tasmania, Australia; Ophthalmology, Department of Surgery, University of Melbourne, East Melbourne, Victoria, Australia.
Burdon KP; Menzies Institute for Medical Research, University of Tasmania, Hobart, Tasmania, Australia; Department of Ophthalmology, Flinders University, Bedford Park, South Australia, Australia. Electronic address: kathryn.burdon@utas.edu.au.

Methods ; 194: 37-47, 2021 10.

Article em En | MEDLINE | ID: mdl-33418061

Assuntos

Catarata; Peixe-Zebra; Animais; Cegueira; Sistemas CRISPR-Cas; Catarata/genética; Edição de Genes; Humanos; Ribonucleoproteínas/genética; Ribonucleoproteínas/metabolismo; Peixe-Zebra/genética; Peixe-Zebra/metabolismo

Palavras-chave

CRISPR; Cas9; Cataract; Genome editing; Ribonucleoprotein (RNP) complex; Zebrafish

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Catarata / Peixe-Zebra Tipo de estudo: Guideline Limite: Animals / Humans Idioma: En Revista: Methods Assunto da revista: BIOQUIMICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Austrália

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