Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes.
STAR Protoc
; 2(1): 100334, 2021 03 19.
Article
em En
| MEDLINE
| ID: mdl-33615277
ABSTRACT
Since the discovery of human induced pluripotent stem cells (hiPSCs), numerous strategies have been established to efficiently derive cardiomyocytes from hiPSCs (hiPSC-CMs). Here, we describe a cost-effective strategy for the subsequent massive expansion (>250-fold) of high-purity hiPSC-CMs relying on two aspects removal of cell-cell contacts and small-molecule inhibition with CHIR99021. The protocol maintains CM functionality, allows cryopreservation, and the cells can be used in downstream assays such as disease modeling, drug and toxicity screening, and cell therapy. For complete details on the use and execution of this protocol, please refer to Buikema (2020).
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Piridinas
/
Pirimidinas
/
Criopreservação
/
Comunicação Celular
/
Células-Tronco Pluripotentes Induzidas
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Holanda