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Optical Manipulation of Subcellular Protein Translocation Using a Photoactivatable Covalent Labeling System.
Kowada, Toshiyuki; Arai, Keisuke; Yoshimura, Akimasa; Matsui, Toshitaka; Kikuchi, Kazuya; Mizukami, Shin.
Afiliação
  • Kowada T; Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Miyagi, 980-8577, Japan.
  • Arai K; Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, 980-8577, Japan.
  • Yoshimura A; Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, 980-8577, Japan.
  • Matsui T; Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  • Kikuchi K; Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Miyagi, 980-8577, Japan.
  • Mizukami S; Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, 980-8577, Japan.
Angew Chem Int Ed Engl ; 60(20): 11378-11383, 2021 05 10.
Article em En | MEDLINE | ID: mdl-33644979
ABSTRACT
The photoactivatable chemically induced dimerization (photo-CID) technique for tag-fused proteins is one of the most promising methods for regulating subcellular protein translocations and protein-protein interactions. However, light-induced covalent protein dimerization in living cells has yet to be established, despite its various advantages. Herein, we developed a photoactivatable covalent protein-labeling technology by applying a caged ligand to the BL-tag system, a covalent protein labeling system that uses mutant ß-lactamase. We further developed CBHD, a caged protein dimerizer, using caged BL-tag and HaloTag ligands, and achieved light-induced protein translocation from the cytoplasm to subcellular regions. In addition, this covalent photo-CID system enabled quick protein translocation to a laser-illuminated microregion. These results indicate that the covalent photo-CID system will expand the scope of CID applications in the optical manipulation of cellular functions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Limite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Limite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Japão