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Isolation and genetic characterization of SARS-CoV-2 from Indian patients in a single family without H/O travel abroad.
Shrivastava, Shubham; Patil, Harshad P; Mhaske, Suhas T; Palkar, Sonali; Lalwani, Sanjay; Mishra, Akhilesh Chandra; Arankalle, Vidya A.
Afiliação
  • Shrivastava S; Department of Communicable Diseases, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed To Be University), Katraj-Dhankawadi, Pune, 411043, India.
  • Patil HP; Department of Communicable Diseases, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed To Be University), Katraj-Dhankawadi, Pune, 411043, India.
  • Mhaske ST; Department of Communicable Diseases, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed To Be University), Katraj-Dhankawadi, Pune, 411043, India.
  • Palkar S; Department of Pediatrics, Bharati Vidyapeeth (Deemed To Be University) Medical College & Hospital, Katraj-Dhankawadi, Pune, 411043, India.
  • Lalwani S; Department of Pediatrics, Bharati Vidyapeeth (Deemed To Be University) Medical College & Hospital, Katraj-Dhankawadi, Pune, 411043, India.
  • Mishra AC; Department of Communicable Diseases, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed To Be University), Katraj-Dhankawadi, Pune, 411043, India.
  • Arankalle VA; Department of Communicable Diseases, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed To Be University), Katraj-Dhankawadi, Pune, 411043, India. varankalle@yahoo.com.
Virus Genes ; 57(3): 245-249, 2021 Jun.
Article em En | MEDLINE | ID: mdl-33683658
In view of the rapidly progressing COVID-19 pandemic, our aim was to isolate and characterize SARS-CoV-2 from Indian patients. SARS-CoV-2 was isolated from nasopharyngeal swabs collected from the two members of a family without any history of (H/O) travel abroad. Both the virus isolates (8003 and 8004) showed CPE on day 3 post-inoculation, viral antigens by immunofluorescence assay and produced distinct, clear and uniform plaques. Infectious virus titers were 5 × 106 and 4 × 106 Pfu/ml by plaque assay and 107.5 and 107 by CPE-based TCID50/ml, respectively. Phylogenetic analysis grouped our isolates with the Italian strains. On comparison with Wuhan strain, 3 unique mutations were identified in nsp3 (A1812D), exonuclease (P1821S) of Orf1ab and spike protein (Q677H) regions, respectively. Both the viruses grouped with Italian strains of SARS-CoV-2 suggesting possible source being the virus imported from Italy. These fully characterized virus isolates will be useful in developing neutralization/virological assays for the evaluation of vaccines/antivirals.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: SARS-CoV-2 / COVID-19 Tipo de estudo: Prognostic_studies Limite: Animals / Humans País/Região como assunto: Asia Idioma: En Revista: Virus Genes Assunto da revista: BIOLOGIA MOLECULAR / VIROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Índia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: SARS-CoV-2 / COVID-19 Tipo de estudo: Prognostic_studies Limite: Animals / Humans País/Região como assunto: Asia Idioma: En Revista: Virus Genes Assunto da revista: BIOLOGIA MOLECULAR / VIROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Índia