High affinity receptors for the bee venom MCD peptide. Quantitative autoradiographic localization at different stages of brain development and relationship with MCD neurotoxicity.

ABSTRACT

High densities of MCD receptors were found in the stratum radiatum of Ammon's horn, the neocortex, the molecular layer of the cerebellum, colliculi and pons. Conversely areas such as the stratum lacunosum moleculare of Ammon's horn contained only low levels of MCD binding sites. The density of MCD receptors is low during the perinatal period and increases rapidly by postnatal day 10 with a decrease of the receptor affinity for MCD. The adult distribution of MCD receptors was reached at postnatal day 30. Increases in density of MCD receptors are discussed in relation with increased neurotoxicity of MCD during brain development. Effects of MCD during the perinatal period are very weak. However, the threshold MCD dose to induce seizures drastically decreased after the first postnatal week. The efficient dose corresponding to adult stage is reached after postnatal day 40.