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Isolation and characterization of Helicobacter suis from human stomach.
Rimbara, Emiko; Suzuki, Masato; Matsui, Hidenori; Nakamura, Masahiko; Morimoto, Misako; Sasakawa, Chihiro; Masuda, Hiroki; Nomura, Sachiyo; Osaki, Takako; Nagata, Noriyo; Shibayama, Keigo; Tokunaga, Kengo.
Afiliação
  • Rimbara E; Department of Bacteriology II, National Institute of Infectious Diseases, 208-0011 Tokyo, Japan; rimbara@nih.go.jp hmatsui@lisci.kitasato-u.ac.jp kentoku@ks.kyorin-u.ac.jp.
  • Suzuki M; Antimicrobial Research Center, National Institute of Infectious Diseases, 189-0002 Tokyo, Japan.
  • Matsui H; Omura Satoshi Memorial Institute, Kitasato University, 108-8641 Tokyo, Japan; rimbara@nih.go.jp hmatsui@lisci.kitasato-u.ac.jp kentoku@ks.kyorin-u.ac.jp.
  • Nakamura M; School of Pharmacy, Kitasato University, 108-8641 Tokyo, Japan.
  • Morimoto M; Department of Research Associate Product Development, Nippon Institute for Biological Science, 198-0024 Tokyo, Japan.
  • Sasakawa C; Department of Research Associate Product Development, Nippon Institute for Biological Science, 198-0024 Tokyo, Japan.
  • Masuda H; Medical Mycology Research Center, Chiba University, 263-8522 Chiba, Japan.
  • Nomura S; Department of Gastrointestinal Surgery, Graduate School of Medicine, The University of Tokyo, 113-8654 Tokyo, Japan.
  • Osaki T; Department of Gastrointestinal Surgery, Nippon Medical University, 113-8602 Tokyo, Japan.
  • Nagata N; Department of Gastrointestinal Surgery, Graduate School of Medicine, The University of Tokyo, 113-8654 Tokyo, Japan.
  • Shibayama K; Department of Infectious Diseases, Kyorin University School of Medicine, 181-8611 Tokyo, Japan.
  • Tokunaga K; Department of Pathology, National Institute of Infectious Diseases, 208-0011 Tokyo, Japan.
Proc Natl Acad Sci U S A ; 118(13)2021 03 30.
Article em En | MEDLINE | ID: mdl-33753513
ABSTRACT
Helicobacter suis, a bacterial species naturally hosted by pigs, can colonize the human stomach in the context of gastric diseases such as gastric mucosa-associated lymphoid tissue (MALT) lymphoma. Because H. suis has been successfully isolated from pigs, but not from humans, evidence linking human H. suis infection to gastric diseases has remained incomplete. In this study, we successfully in vitro cultured H. suis directly from human stomachs. Unlike Helicobacter pylori, the viability of H. suis decreases significantly on neutral pH; therefore, we achieved this using a low-pH medium for transport of gastric biopsies. Ultimately, we isolated H. suis from three patients with gastric diseases, including gastric MALT lymphoma. Successful eradication of H. suis yielded significant improvements in endoscopic and histopathological findings. Oral infection of mice with H. suis clinical isolates elicited gastric and systemic inflammatory responses; in addition, progression of gastric mucosal metaplasia was observed 4 mo postinfection. Because H. suis could be isolated from the stomachs of infected mice, our findings satisfied Koch's postulates. Although further prospective clinical studies are needed, H. suis, like H. pylori, is likely a gastric pathogen in humans. Furthermore, comparative genomic analysis of H. suis using complete genomes of clinical isolates revealed that the genome of each H. suis isolate contained highly plastic genomic regions encoding putative strain-specific virulence factors, including type IV secretion system-associated genes, and that H. suis isolates from humans and pigs were genetically very similar, suggesting possible pig-to-human transmission.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estômago / Gastropatias / Infecções por Helicobacter / Helicobacter heilmannii / Fatores de Virulência Tipo de estudo: Prognostic_studies Limite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estômago / Gastropatias / Infecções por Helicobacter / Helicobacter heilmannii / Fatores de Virulência Tipo de estudo: Prognostic_studies Limite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2021 Tipo de documento: Article