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An investigation of the environmental niches of blown pack spoilage causing Clostridium estertheticum and Clostridium gasigenes on New Zealand beef and sheep farms.
Esteves, Eden; Gupta, Tanushree B; Whyte, Paul; Brightwell, Gale; Bolton, Declan.
Afiliação
  • Esteves E; Department of Food Safety, Teagasc Food Research Centre, Ashtown, Dublin, 15, Ireland; School of Veterinary Medicine, UCD, Belfield, Dublin, 4, Ireland; Food Assurance Team, Hopkirk Research Institute, AgResearch Ltd, Massey University, Palmerston North, 4472, New Zealand.
  • Gupta TB; Food Assurance Team, Hopkirk Research Institute, AgResearch Ltd, Massey University, Palmerston North, 4472, New Zealand. Electronic address: tanushree.gupta@agresearch.co.nz.
  • Whyte P; School of Veterinary Medicine, UCD, Belfield, Dublin, 4, Ireland.
  • Brightwell G; Food Assurance Team, Hopkirk Research Institute, AgResearch Ltd, Massey University, Palmerston North, 4472, New Zealand; New Zealand Food Safety and Science Centre, Massey University, Palmerston North, 4472, New Zealand.
  • Bolton D; Department of Food Safety, Teagasc Food Research Centre, Ashtown, Dublin, 15, Ireland.
Food Microbiol ; 98: 103769, 2021 Sep.
Article em En | MEDLINE | ID: mdl-33875205
ABSTRACT
The transfer of blown pack spoilage causing Clostridium spores from the farm to the meat plant is of growing concern to the meat industry. This study investigated the environmental niches of these Clostridium spp., specifically Clostridium estertheticum and Clostridium gasigenes in the beef and sheep farm environments in New Zealand. Faecal, soil, grass, drinking water, puddle water and feed (fodder beet, hay, bailage and silage, where available) samples were collected on five beef and sheep farms during Winter and Spring in 2018, in North and South Island, respectively. Beef and sheep farm samples were tested for C. estertheticum and C. gasigenes using enrichment plus PCR, qPCR and direct plating. C. estertheticum was detected in bovine faecal (4%), soil (2-18%) and grass (0-12%) samples at concentration of up to 2.0 log10 cfu/g. C. gasigenes were found in 18-46% of faecal, 16-82% of soil, 12-44% of grass, 0-44.4% of drinking water and 0-58.3% of puddle water samples tested and the direct counts ranged from 2.4 log10 cfu/ml in puddle water to 3.4 log10 cfu/g in soil. C. estertheticum were detected by qPCR in sheep farms in ovine feces (2.3%), soil (2.3%) and fodder beet (10%). All other sample types (grass, drinking water, puddle water, baleage, hay, silage and fodder beet) were negative using direct and enrichment plus PCR methods. In contrast C. gasigenes was detected in of faecal (22.7-38.6%), soil (22.7-84.1%), grass (17.5-34.1%) drinking water (35.7-78.6%), puddle water (33.3-40%), hay baleage (57%), silage (2%) and fodder beet (10%) at concentrations of up to 3.7 log10 cfu/g/ml. It was concluded that C. estertheticum and C. gasigenes were common on beef and sheep farms with the latter having higher incidence and mean concentration.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Clostridium / Microbiologia Ambiental / Carne Limite: Animals País/Região como assunto: Oceania Idioma: En Revista: Food Microbiol Assunto da revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Nova Zelândia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Clostridium / Microbiologia Ambiental / Carne Limite: Animals País/Região como assunto: Oceania Idioma: En Revista: Food Microbiol Assunto da revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Nova Zelândia