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The emerging landscape of single-molecule protein sequencing technologies.
Alfaro, Javier Antonio; Bohländer, Peggy; Dai, Mingjie; Filius, Mike; Howard, Cecil J; van Kooten, Xander F; Ohayon, Shilo; Pomorski, Adam; Schmid, Sonja; Aksimentiev, Aleksei; Anslyn, Eric V; Bedran, Georges; Cao, Chan; Chinappi, Mauro; Coyaud, Etienne; Dekker, Cees; Dittmar, Gunnar; Drachman, Nicholas; Eelkema, Rienk; Goodlett, David; Hentz, Sébastien; Kalathiya, Umesh; Kelleher, Neil L; Kelly, Ryan T; Kelman, Zvi; Kim, Sung Hyun; Kuster, Bernhard; Rodriguez-Larrea, David; Lindsay, Stuart; Maglia, Giovanni; Marcotte, Edward M; Marino, John P; Masselon, Christophe; Mayer, Michael; Samaras, Patroklos; Sarthak, Kumar; Sepiashvili, Lusia; Stein, Derek; Wanunu, Meni; Wilhelm, Mathias; Yin, Peng; Meller, Amit; Joo, Chirlmin.
Afiliação
  • Alfaro JA; International Centre for Cancer Vaccine Science, University of Gdansk, Gdansk, Poland. javier.alfaro@proteogenomics.ca.
  • Bohländer P; Faculty of Applied Sciences, Delft University of Technology, Delft, the Netherlands.
  • Dai M; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
  • Filius M; Department of Systems Biology, Harvard Medical School, Boston, MA, USA.
  • Howard CJ; Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands.
  • van Kooten XF; Department of Chemistry, University of Texas at Austin, Austin, TX, USA.
  • Ohayon S; Department of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
  • Pomorski A; Department of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
  • Schmid S; Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands.
  • Aksimentiev A; NanoDynamicsLab, Laboratory of Biophysics, Wageningen University, Wageningen, the Netherlands.
  • Anslyn EV; Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
  • Bedran G; Department of Chemistry, University of Texas at Austin, Austin, TX, USA.
  • Cao C; International Centre for Cancer Vaccine Science, University of Gdansk, Gdansk, Poland.
  • Chinappi M; Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Coyaud E; Dipartimento di Ingegneria Industriale, Università di Roma Tor Vergata, Rome, Italy.
  • Dekker C; Univ. Lille, Inserm, CHU Lille, U1192-Protéomique Réponse Inflammatoire Spectrométrie de Masse-PRISM, Lille, France.
  • Dittmar G; Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands.
  • Drachman N; Department of Infection and Immunity, Luxembourg Institute of Health, Strassen, Luxembourg.
  • Eelkema R; Department of Life Sciences and Medicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg.
  • Goodlett D; Department of Physics, Brown University, Providence, RI, USA.
  • Hentz S; Faculty of Applied Sciences, Delft University of Technology, Delft, the Netherlands.
  • Kalathiya U; International Centre for Cancer Vaccine Science, University of Gdansk, Gdansk, Poland.
  • Kelleher NL; Genome BC Proteomics Centre, University of Victoria, Victoria, British Columbia, Canada.
  • Kelly RT; Université Grenoble Alpes, CEA, LETI, Grenoble, France.
  • Kelman Z; International Centre for Cancer Vaccine Science, University of Gdansk, Gdansk, Poland.
  • Kim SH; Departments of Chemistry and Molecular Biosciences, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Kuster B; Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.
  • Rodriguez-Larrea D; Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology, University of Maryland, Rockville, MD, USA.
  • Lindsay S; Biomolecular Labeling Laboratory, Institute for Bioscience and Biotechnology Research, Rockville, MD, USA.
  • Maglia G; Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands.
  • Marcotte EM; Chair of Proteomics and Bioanalytics, Technische Universität München, Freising, Germany.
  • Marino JP; Bavarian Center for Biomolecular Mass Spectrometry, Freising, Germany.
  • Masselon C; Department of Biochemistry and Molecular Biology, Biofisika Institute (CSIC, UPV/EHU), Leioa, Spain.
  • Mayer M; Biodesign Institute, School of Molecular Sciences, Department of Physics, Arizona State University, Tempe, AZ, USA.
  • Samaras P; Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands.
  • Sarthak K; Department of Molecular Biosciences, Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX, USA.
  • Sepiashvili L; Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology, University of Maryland, Rockville, MD, USA.
  • Stein D; Université Grenoble Alpes, CEA, Inserm, BGE U1038, Grenoble, France.
  • Wanunu M; Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland.
  • Wilhelm M; Chair of Proteomics and Bioanalytics, Technische Universität München, Freising, Germany.
  • Yin P; Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
  • Meller A; University of Toronto, Hospital for Sick Children, Toronto, Ontario, Canada.
  • Joo C; Department of Physics, Brown University, Providence, RI, USA.
Nat Methods ; 18(6): 604-617, 2021 06.
Article em En | MEDLINE | ID: mdl-34099939
ABSTRACT
Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise de Sequência de Proteína / Imagem Individual de Molécula Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Polônia
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise de Sequência de Proteína / Imagem Individual de Molécula Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Polônia