The advancements, challenges, and future implications of the CRISPR/Cas9 system in swine research.

Zhang, Jinfu; Khazalwa, Emmanuel M; Abkallo, Hussein M; Zhou, Yuan; Nie, Xiongwei; Ruan, Jinxue; Zhao, Changzhi; Wang, Jieru; Xu, Jing; Li, Xinyun; Zhao, Shuhong; Zuo, Erwei; Steinaa, Lucilla; Xie, Shengsong

Zhang, Jinfu; Khazalwa, Emmanuel M; Abkallo, Hussein M; Zhou, Yuan; Nie, Xiongwei; Ruan, Jinxue; Zhao, Changzhi; Wang, Jieru; Xu, Jing; Li, Xinyun; Zhao, Shuhong; Zuo, Erwei; Steinaa, Lucilla; Xie, Shengsong.

Afiliação

Zhang J; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, PR China.
Khazalwa EM; Animal and Human Health Program, Biosciences, International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi 00100, Kenya.
Abkallo HM; Animal and Human Health Program, Biosciences, International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi 00100, Kenya.
Zhou Y; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, PR China.
Nie X; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, PR China.
Ruan J; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, PR China.
Zhao C; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, PR China.
Wang J; Key Laboratory of Pig Molecular Quantitative Genetics of Anhui Academy of Agricultural Sciences, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety Engineering, Institute of Animal Husbandry and Veterinar
Xu J; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, PR China.
Li X; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, PR China; The Cooperative Innovation Center for Sustainable Pi
Zhao S; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, PR China; The Cooperative Innovation Center for Sustainable Pi
Zuo E; Lingnan Guangdong Laboratory of Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, PR China. Electronic address: erweizuo@163.com.
Steinaa L; Animal and Human Health Program, Biosciences, International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi 00100, Kenya. Electronic address: l.steinaa@cgiar.org.
Xie S; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, PR China; Animal and Human Health Program, Biosciences, Intern

J Genet Genomics ; 48(5): 347-360, 2021 05 20.

Article em En | MEDLINE | ID: mdl-34144928

ABSTRACT

ABSTRACT

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) genome editing technology has dramatically influenced swine research by enabling the production of high-quality disease-resistant pig breeds, thus improving yields. In addition, CRISPR/Cas9 has been used extensively in pigs as one of the tools in biomedical research. In this review, we present the advancements of the CRISPR/Cas9 system in swine research, such as animal breeding, vaccine development, xenotransplantation, and disease modeling. We also highlight the current challenges and some potential applications of the CRISPR/Cas9 technologies.

Assuntos

Sistemas CRISPR-Cas; Edição de Genes/métodos; Edição de Genes/tendências; Pesquisa/tendências; Animais; Animais Geneticamente Modificados; Cruzamento; Resistência à Doença; Estudos de Associação Genética/métodos; Estudos de Associação Genética/tendências; Engenharia Genética/métodos; Engenharia Genética/tendências; Humanos; Modelos Animais; Locos de Características Quantitativas; Característica Quantitativa Herdável; Suínos

Palavras-chave

Base editing; CRISPR screening; CRISPR/Cas9; Genome editing; Swine

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pesquisa / Sistemas CRISPR-Cas / Edição de Genes Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: J Genet Genomics Ano de publicação: 2021 Tipo de documento: Article

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