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Dynamic changes in mitochondrial 3D structure during folliculogenesis and luteal formation in the goat large luteal cell lineage.
Jiang, Yi-Fan; Yu, Pin-Huan; Budi, Yovita Permata; Chiu, Chih-Hsien; Fu, Chi-Yu.
Afiliação
  • Jiang YF; Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Rm. 104-1, No.1, Sec. 4, Roosevelt Road, Taipei City, 10617, Taiwan, ROC. yfjiang@ntu.edu.tw.
  • Yu PH; Institute of Veterinary Clinical Science, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan, ROC.
  • Budi YP; Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Rm. 104-1, No.1, Sec. 4, Roosevelt Road, Taipei City, 10617, Taiwan, ROC.
  • Chiu CH; Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan, ROC.
  • Fu CY; Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan, ROC.
Sci Rep ; 11(1): 15564, 2021 07 30.
Article em En | MEDLINE | ID: mdl-34330986
ABSTRACT
In mammalian ovaries, mitochondria are integral sites of energy production and steroidogenesis. While shifts in cellular activities and steroidogenesis are well characterized during the differentiation of large luteal cells in folliculogenesis and luteal formation, mitochondrial dynamics during this process have not been previously evaluated. In this study, we collected ovaries containing primordial follicles, mature follicles, corpus hemorrhagicum, or corpus luteum from goats at specific times in the estrous cycle. Enzyme histochemistry, ultrastructural observations, and 3D structural analysis of serial sections of mitochondria revealed that branched mitochondrial networks were predominant in follicles, while spherical and tubular mitochondria were typical in large luteal cells. Furthermore, the average mitochondrial diameter and volume increased from folliculogenesis to luteal formation. In primordial follicles, the signals of cytochrome c oxidase and ATP synthase were undetectable in most cells, and the large luteal cells from the corpus hemorrhagicum also showed low enzyme signals and content when compared with granulosa cells in mature follicles or large luteal cells from the corpus luteum. Our findings suggest that the mitochondrial enlargement could be an event during folliculogenesis and luteal formation, while the modulation of mitochondrial morphology and respiratory enzyme expressions may be related to tissue remodeling during luteal formation.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lipogênese / Células Lúteas Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lipogênese / Células Lúteas Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2021 Tipo de documento: Article