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Systematic phenotyping and characterization of the 5xFAD mouse model of Alzheimer's disease.
Forner, Stefania; Kawauchi, Shimako; Balderrama-Gutierrez, Gabriela; Kramár, Enikö A; Matheos, Dina P; Phan, Jimmy; Javonillo, Dominic I; Tran, Kristine M; Hingco, Edna; da Cunha, Celia; Rezaie, Narges; Alcantara, Joshua A; Baglietto-Vargas, David; Jansen, Camden; Neumann, Jonathan; Wood, Marcelo A; MacGregor, Grant R; Mortazavi, Ali; Tenner, Andrea J; LaFerla, Frank M; Green, Kim N.
Afiliação
  • Forner S; Institute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.
  • Kawauchi S; Transgenic Mouse Facility, University Laboratory Animal Resources, Office of Research, University of California, Irvine, CA, 92697, USA.
  • Balderrama-Gutierrez G; Department of Developmental and Cell Biology, University of California, Irvine, CA, 92697, USA.
  • Kramár EA; Center for Complex Biological Systems, University of California, Irvine, CA, 92697, USA.
  • Matheos DP; Department of Neurobiology and Behavior, University of California, Irvine, CA, 92697, USA.
  • Phan J; Department of Neurobiology and Behavior, University of California, Irvine, CA, 92697, USA.
  • Javonillo DI; Institute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.
  • Tran KM; Institute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.
  • Hingco E; Institute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.
  • da Cunha C; Institute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.
  • Rezaie N; Institute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.
  • Alcantara JA; Department of Developmental and Cell Biology, University of California, Irvine, CA, 92697, USA.
  • Baglietto-Vargas D; Center for Complex Biological Systems, University of California, Irvine, CA, 92697, USA.
  • Jansen C; Transgenic Mouse Facility, University Laboratory Animal Resources, Office of Research, University of California, Irvine, CA, 92697, USA.
  • Neumann J; Institute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.
  • Wood MA; Department of Developmental and Cell Biology, University of California, Irvine, CA, 92697, USA.
  • MacGregor GR; Center for Complex Biological Systems, University of California, Irvine, CA, 92697, USA.
  • Mortazavi A; Transgenic Mouse Facility, University Laboratory Animal Resources, Office of Research, University of California, Irvine, CA, 92697, USA.
  • Tenner AJ; Department of Neurobiology and Behavior, University of California, Irvine, CA, 92697, USA.
  • LaFerla FM; Transgenic Mouse Facility, University Laboratory Animal Resources, Office of Research, University of California, Irvine, CA, 92697, USA.
  • Green KN; Department of Developmental and Cell Biology, University of California, Irvine, CA, 92697, USA.
Sci Data ; 8(1): 270, 2021 10 15.
Article em En | MEDLINE | ID: mdl-34654824
ABSTRACT
Mouse models of human diseases are invaluable tools for studying pathogenic mechanisms and testing interventions and therapeutics. For disorders such as Alzheimer's disease in which numerous models are being generated, a challenging first step is to identify the most appropriate model and age to effectively evaluate new therapeutic approaches. Here we conducted a detailed phenotypic characterization of the 5xFAD model on a congenic C57BL/6 J strain background, across its lifespan - including a seldomly analyzed 18-month old time point to provide temporally correlated phenotyping of this model and a template for characterization of new models of LOAD as they are generated. This comprehensive analysis included quantification of plaque burden, Aß biochemical levels, and neuropathology, neurophysiological measurements and behavioral and cognitive assessments, and evaluation of microglia, astrocytes, and neurons. Analysis of transcriptional changes was conducted using bulk-tissue generated RNA-seq data from microdissected cortices and hippocampi as a function of aging, which can be explored at the MODEL-AD Explorer and AD Knowledge Portal. This deep-phenotyping pipeline identified novel aspects of age-related pathology in the 5xFAD model.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenótipo / Modelos Animais de Doenças / Doença de Alzheimer Limite: Animals Idioma: En Revista: Sci Data Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenótipo / Modelos Animais de Doenças / Doença de Alzheimer Limite: Animals Idioma: En Revista: Sci Data Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos