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Characterization of Streptomyces sp. LS462 with high productivity of echinomycin, a potent antituberculosis and synergistic antifungal antibiotic.
Chen, Caixia; Chen, Xiangyin; Ren, Biao; Guo, Hui; Abdel-Mageed, Wael M; Liu, Xueting; Song, Fuhang; Zhang, Lixin.
Afiliação
  • Chen C; Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P. R. China.
  • Chen X; School of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA.
  • Ren B; State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, P. R. China.
  • Guo H; Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P. R. China.
  • Abdel-Mageed WM; West China Hospital of Stomatology, Sichuan University, Sichuan 610041, P. R. China.
  • Liu X; Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P. R. China.
  • Song F; Institute of Biophysics, Chinese Academy of Sciences, Beijing 100190, P. R. China.
  • Zhang L; Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P. R. China.
J Ind Microbiol Biotechnol ; 48(9-10)2021 Dec 23.
Article em En | MEDLINE | ID: mdl-34661655
A biologically active microbial strain, designated as "LS462," was isolated from a soil sample collected from Yaoli Virgin Forest of Jiangxi Province, China. The strain was able to produce a high yield of echinomycin (172 mg/l) even under nonoptimized culture conditions and is proposed to serve as a promising source of echinomycin. In this study, echinomycin exhibited strong anti-Mycobacterium tuberculosis H37Rv activity and synergistic antifungal effect with a greatly reduced dosage of posaconazole on Candida albicans SC5314. The strain belongs to the genus Streptomyces according to its morphological and 16S rDNA phylogenetic analysis. The 16S rDNA was found to have the highest sequence identity with Streptomyces fuscichromogenes (99.37% similarity). Extensive nuclear magnetic resonance and mass spectroscopic data were used to determine the structure of echinomycin. The strain S. fuscichromogenes has not been previously reported to produce echinomycin. Strain LS462 may be exploited as a new potential source for the commercial production of echinomycin. Also, this work is the first to report the new synergistic antifungal activity of echinomycin and further study of the synergistic mechanism will be helpful to guide the development of antifungal agents.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptomyces / Equinomicina / Antibióticos Antituberculose / Antifúngicos País/Região como assunto: Asia Idioma: En Revista: J Ind Microbiol Biotechnol Assunto da revista: BIOTECNOLOGIA / MICROBIOLOGIA Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptomyces / Equinomicina / Antibióticos Antituberculose / Antifúngicos País/Região como assunto: Asia Idioma: En Revista: J Ind Microbiol Biotechnol Assunto da revista: BIOTECNOLOGIA / MICROBIOLOGIA Ano de publicação: 2021 Tipo de documento: Article