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Passive coupling of membrane tension and cell volume during active response of cells to osmosis.
Roffay, Chloé; Molinard, Guillaume; Kim, Kyoohyun; Urbanska, Marta; Andrade, Virginia; Barbarasa, Victoria; Nowak, Paulina; Mercier, Vincent; García-Calvo, José; Matile, Stefan; Loewith, Robbie; Echard, Arnaud; Guck, Jochen; Lenz, Martin; Roux, Aurélien.
Afiliação
  • Roffay C; Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
  • Molinard G; Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
  • Kim K; Max Planck Institute for the Science of Light, DE-91058 Erlangen, Germany.
  • Urbanska M; Biotechnology Center, Technische Universität Dresden, D-01307 Dresden, Germany.
  • Andrade V; Max Planck Institute for the Science of Light, DE-91058 Erlangen, Germany.
  • Barbarasa V; Biotechnology Center, Technische Universität Dresden, D-01307 Dresden, Germany.
  • Nowak P; Membrane Traffic and Cell Division Laboratory, Institut Pasteur, Université de Paris, CNRS UMR3691, F-75015 Paris, France.
  • Mercier V; Collège Doctoral, Sorbonne Université, F-75005 Paris, France.
  • García-Calvo J; Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
  • Matile S; Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
  • Loewith R; Department of Molecular Biology, University of Geneva, CH-1211 Geneva, Switzerland.
  • Echard A; Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
  • Guck J; Department of Organic Chemistry, University of Geneva, CH-1211 Geneva, Switzerland.
  • Lenz M; Department of Organic Chemistry, University of Geneva, CH-1211 Geneva, Switzerland.
  • Roux A; National Centre of Competence in Research Chemical Biology, University of Geneva, CH-1211 Geneva, Switzerland.
Proc Natl Acad Sci U S A ; 118(47)2021 11 23.
Article em En | MEDLINE | ID: mdl-34785592
ABSTRACT
During osmotic changes of their environment, cells actively regulate their volume and plasma membrane tension that can passively change through osmosis. How tension and volume are coupled during osmotic adaptation remains unknown, as their quantitative characterization is lacking. Here, we performed dynamic membrane tension and cell volume measurements during osmotic shocks. During the first few seconds following the shock, cell volume varied to equilibrate osmotic pressures inside and outside the cell, and membrane tension dynamically followed these changes. A theoretical model based on the passive, reversible unfolding of the membrane as it detaches from the actin cortex during volume increase quantitatively describes our data. After the initial response, tension and volume recovered from hypoosmotic shocks but not from hyperosmotic shocks. Using a fluorescent membrane tension probe (fluorescent lipid tension reporter [Flipper-TR]), we investigated the coupling between tension and volume during these asymmetric recoveries. Caveolae depletion and pharmacological inhibition of ion transporters and channels, mTORCs, and the cytoskeleton all affected tension and volume responses. Treatments targeting mTORC2 and specific downstream effectors caused identical changes to both tension and volume responses, their coupling remaining the same. This supports that the coupling of tension and volume responses to osmotic shocks is primarily regulated by mTORC2.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osmose / Tamanho Celular / Membranas Limite: Humans Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osmose / Tamanho Celular / Membranas Limite: Humans Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Suíça