Concordance of acquired mutations between metastatic lesions and liquid biopsy in metastatic colorectal cancer.
Future Sci OA
; 7(10): FSO757, 2021 Dec.
Article
em En
| MEDLINE
| ID: mdl-34840814
ABSTRACT
AIM:
To evaluate whether PCR-reverse sequence-specific oligonucleotide can examine the concordance between liquid biopsy and metastatic lesions with acquired resistance. MATERIALS &METHODS:
We examined acquired mutations in chemoresistant lesions and blood obtained from four patients with RAS wild-type metastatic colorectal cancer who underwent treatment with anti-epidermal growth factor receptor antibodies.RESULTS:
In one patient, metastatic lesions harbored diverse acquired mutations in KRAS in all seven metastases; the two acquired mutations were detectable in blood collected after the patient acquired resistance. None of the other patients exhibited liquid biopsy mutations, except one, with a BRAF mutation confirmed in primary tumor and peritoneal dissemination.CONCLUSION:
Liquid biopsy based on PCR-reverse sequence-specific oligonucleotide is a successful procedure for capturing acquired mutations with precise information on the RAS mutational spectrum.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Future Sci OA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Japão