Effect of Divalent Metal Ion on the Structure, Stability and Function of <i>Klebsiella pneumoniae</i> Nicotinate-Nucleotide Adenylyltransferase: Empirical and Computational Studies.

Jeje, Olamide; Maake, Reabetswe; van Deventer, Ruan; Esau, Veruschka; Iwuchukwu, Emmanuel Amarachi; Meyer, Vanessa; Khoza, Thandeka; Achilonu, Ikechukwu

Effect of Divalent Metal Ion on the Structure, Stability and Function of Klebsiella pneumoniae Nicotinate-Nucleotide Adenylyltransferase: Empirical and Computational Studies.

Jeje, Olamide; Maake, Reabetswe; van Deventer, Ruan; Esau, Veruschka; Iwuchukwu, Emmanuel Amarachi; Meyer, Vanessa; Khoza, Thandeka; Achilonu, Ikechukwu.

Afiliação

Jeje O; Protein Structure-Function Research Unit, School of Molecular and Cell Biology, Faculty of Science, University of the Witwatersrand, Johannesburg 2050, South Africa.
Maake R; Protein Structure-Function Research Unit, School of Molecular and Cell Biology, Faculty of Science, University of the Witwatersrand, Johannesburg 2050, South Africa.
van Deventer R; Protein Structure-Function Research Unit, School of Molecular and Cell Biology, Faculty of Science, University of the Witwatersrand, Johannesburg 2050, South Africa.
Esau V; Protein Structure-Function Research Unit, School of Molecular and Cell Biology, Faculty of Science, University of the Witwatersrand, Johannesburg 2050, South Africa.
Iwuchukwu EA; Protein Structure-Function Research Unit, School of Molecular and Cell Biology, Faculty of Science, University of the Witwatersrand, Johannesburg 2050, South Africa.
Meyer V; Functional Genomics and Immunogenetics Laboratory, School of Molecular and Cell Biology, Faculty of Science, University of the Witwatersrand, Johannesburg 2050, South Africa.
Khoza T; Department of Biochemistry, School of Life Sciences, Pietermaritzburg Campus, University of KwaZulu-Natal, Pietermaritzburg 3209, South Africa.
Achilonu I; Protein Structure-Function Research Unit, School of Molecular and Cell Biology, Faculty of Science, University of the Witwatersrand, Johannesburg 2050, South Africa.

Int J Mol Sci ; 23(1)2021 Dec 23.

Article em En | MEDLINE | ID: mdl-35008542

Assuntos

Proteínas de Bactérias/química; Proteínas de Bactérias/metabolismo; Cátions Bivalentes/metabolismo; Klebsiella pneumoniae/metabolismo; Nicotinamida-Nucleotídeo Adenililtransferase/química; Nicotinamida-Nucleotídeo Adenililtransferase/metabolismo; Sítios de Ligação/fisiologia; Simulação por Computador; Cristalografia por Raios X/métodos; Simulação de Acoplamento Molecular/métodos; NAD/metabolismo; Mononucleotídeo de Nicotinamida/análogos & derivados; Mononucleotídeo de Nicotinamida/metabolismo

Palavras-chave

ATP; Klebsiella pneumoniae; NAD+; cations; computational modelling; nicotinate nucleotide adenylyltransferase (NNAT); protein expression; stability; thermal shift assay; ß nicotinamide mononucleotide

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Cátions Bivalentes / Klebsiella pneumoniae / Nicotinamida-Nucleotídeo Adenililtransferase Idioma: En Revista: Int J Mol Sci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: África do Sul

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