Easy-to-Prepare Mini-Chemosensor Array for Simultaneous Detection of Cysteine and Glutathione Derivatives.
ACS Appl Bio Mater
; 4(3): 2113-2119, 2021 03 15.
Article
em En
| MEDLINE
| ID: mdl-35014339
ABSTRACT
We herein propose an easy-to-prepare mini-chemosensor array constructed by two-types of off-the-shelf coumarin dyes for simultaneous classification and quantification of sulfur-containing amino acids (SCAAs) (i.e., l-cysteine, l-cystine, l-homocysteine, glutathione, and glutathione disulfide). The detection mechanism of SCAAs relied on a coordination-based sensor array (CBSA) utilizing the competitive binding among the coumarin dye, a Zn2+ ion and SCAAs. The reversible feature of the coordination bond of the coumarin-Zn2+ resulted in UV/vis spectral shifts and fluorescence enhancement in response to the analytes, offering the multianalyte detection in high accuracy. Furthermore, a mixture of glutathione and l-cysteine was successfully quantified in a sample containing human blood serum. This study would be an important example of the optical chemosensor array toward the rapid and accurate detection of biomarkers.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Materiais Biocompatíveis
/
Cisteína
/
Corantes Fluorescentes
/
Glutationa
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
ACS Appl Bio Mater
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Japão