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Protective Effect of Shenfu Injection on Vascular Endothelial Damage in a Porcine Model of Hemorrhagic Shock.
Zhang, Ming-Qing; Zhang, Qiang; Yuan, Wei; Wu, Jun-Yuan; Liang, Yong; Qin, Hong-Jie; Li, Chun-Sheng.
Afiliação
  • Zhang MQ; Department of Emergency Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
  • Zhang Q; Department of Critical Care Medicine, Peking University Third Hospital, Beijing, 100191, China.
  • Yuan W; Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.
  • Wu JY; Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.
  • Liang Y; Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.
  • Qin HJ; Department of Emergency Medicine, Beijing Luhe Hospital, Capital Medical University, Beijing, 101199, China.
  • Li CS; Department of Emergency Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China. lcscyyy@163.com.
Chin J Integr Med ; 28(9): 794-801, 2022 Sep.
Article em En | MEDLINE | ID: mdl-35023060
OBJECTIVE: To investigate the effects of Shenfu Injection (, SFI) on endothelial damage in a porcine model of hemorrhagic shock (HS). METHODS: After being bled to a mean arterial pressure of 40±3 mm Hg and held for 60 min, 32 pigs were treated with a venous injection of either shed blood (transfusion group), shed blood and saline (saline group), shed blood and SFI (SFI group) or without resuscitation (sham group). Venous blood samples were collected and analyzed at baseline and 0, 1, 2, 4, and 6 h after HS. Tumor necrosis factor-α (TNF-α), serum interleuking (IL)-6, and IL-10 levels were measured by enzyme-linked immunosorbent assay (ELISA); expressions of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule 1 (ICAM -1), von Willebrand factor (vWF), plasminogen activator inhibitor-1 (PAI-1) and Bcl-2, Bax, and caspase-3 proteins were determined by Western blot. RESULTS: The serum level of TNF-α in the SFI group was significantly lower than in the other groups at 0, 1, and 2 h after HS, while the level of IL-6 was lower at 4 and 6 h compared with the saline group (P<0.01 or P<0.05). The concentration of serum IL-10 was significantly higher in the SFI group than in the other groups at 0, 1, 4, and 6 h after HS (P<0.01). Western blot and immunohistochemistry of vascular tissue showed that the expression of caspase-3 was downregulated, and that of Bcl-2 and Bax was upregulated in the SFI group compared to other groups (P<0.05). CONCLUSION: SFI attenuated endothelial injury in the porcine model of HS by inhibiting cell apoptosis, suppressing the formation of proinflammatory cytokines, and reducing endothelial activation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Choque Hemorrágico / Fator de Necrose Tumoral alfa Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Chin J Integr Med Assunto da revista: TERAPIAS COMPLEMENTARES Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Choque Hemorrágico / Fator de Necrose Tumoral alfa Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Chin J Integr Med Assunto da revista: TERAPIAS COMPLEMENTARES Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China