Vanadate as a new substrate for nucleoside phosphorylases.

Antipov, Alexey N; Okorokova, Natalya A; Safonova, Tatyana N; Veiko, Vladimir P

Antipov, Alexey N; Okorokova, Natalya A; Safonova, Tatyana N; Veiko, Vladimir P.

Afiliação

Antipov AN; A.N. Bach Institute of Biochemistry, Federal Research Centre of Biotechnology of the Russian Academy of Science, Leninsky Prosp. 33, Build 2, Moscow, 119071, Russia. a_antipov@hotbox.ru.
Okorokova NA; A.N. Bach Institute of Biochemistry, Federal Research Centre of Biotechnology of the Russian Academy of Science, Leninsky Prosp. 33, Build 2, Moscow, 119071, Russia.
Safonova TN; A.N. Bach Institute of Biochemistry, Federal Research Centre of Biotechnology of the Russian Academy of Science, Leninsky Prosp. 33, Build 2, Moscow, 119071, Russia.
Veiko VP; A.N. Bach Institute of Biochemistry, Federal Research Centre of Biotechnology of the Russian Academy of Science, Leninsky Prosp. 33, Build 2, Moscow, 119071, Russia.

J Biol Inorg Chem ; 27(2): 221-227, 2022 03.

Article em En | MEDLINE | ID: mdl-35094116

ABSTRACT

ABSTRACT

Orthovanadate was shown to serve as a substrate for nucleoside phosphorylases from Escherichia coli, Shewanella oneidensis, Geobacillus stearothermophilus, and Halomonas chromatireducens AGD 8-3. An exception is thymidine phosphorylase from the extremophilic haloalkaliphilic bacterium Halomonas chromatireducens AGD 8-3, which cannot catalyze the vanadolysis of nucleosides. The kinetic parameters of nucleoside vanadolysis were evaluated.

Assuntos

Nucleosídeos; Vanadatos; Escherichia coli/metabolismo; Halomonas; Pentosiltransferases/química; Pentosiltransferases/metabolismo; Especificidade por Substrato

Palavras-chave

Enzyme catalysis; Inhibition; Nucleoside phosphorylase; Vanadate; Vanadolysis

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vanadatos / Nucleosídeos Idioma: En Revista: J Biol Inorg Chem Assunto da revista: BIOQUIMICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Federação Russa

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