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Adult plant stem rust resistance in durum wheat Glossy Huguenot: mapping, marker development and validation.
Mago, Rohit; Chen, Chunhong; Xia, Xiaodi; Whan, Alex; Forrest, Kerrie; Basnet, Bhoja R; Perera, Geetha; Chandramohan, Sutha; Randhawa, Mandeep; Hayden, Matthew; Bansal, Urmil; Huerta-Espino, Julio; Singh, Ravi P; Bariana, Harbans; Lagudah, Evans.
Afiliação
  • Mago R; CSIRO Agriculture and Food, P.O. Box 1700, Canberra, ACT, 2601, Australia. rohit.mago@csiro.au.
  • Chen C; CSIRO Agriculture and Food, P.O. Box 1700, Canberra, ACT, 2601, Australia.
  • Xia X; CSIRO Agriculture and Food, P.O. Box 1700, Canberra, ACT, 2601, Australia.
  • Whan A; CSIRO Agriculture and Food, P.O. Box 1700, Canberra, ACT, 2601, Australia.
  • Forrest K; Agriculture Victoria Research, Department of Jobs, Precincts and Regions, Agribio, 5 Ring Rd, Bundoora, VIC, 3083, Australia.
  • Basnet BR; CIMMYT, Carretera Mexico-Veracruz Km 18, El Batan, Texcoco, Estado de México, Mexico.
  • Perera G; CSIRO Agriculture and Food, P.O. Box 1700, Canberra, ACT, 2601, Australia.
  • Chandramohan S; CSIRO Agriculture and Food, P.O. Box 1700, Canberra, ACT, 2601, Australia.
  • Randhawa M; ICRAF House, CIMMYT Kenya, United Nations Avenue, Gigiri, Village Market, P.O. Box 1041, 00621, Nairobi, Kenya.
  • Hayden M; Agriculture Victoria Research, Department of Jobs, Precincts and Regions, Agribio, 5 Ring Rd, Bundoora, VIC, 3083, Australia.
  • Bansal U; Faculty of Science, School of Life and Environmental Sciences, The University of Sydney Plant Breeding Institute, 107 Cobbitty Road, Cobbitty, NSW, 2570, Australia.
  • Huerta-Espino J; Campo Experimental Valle de México, INIFAP, Chapingo, Estado de México, Mexico.
  • Singh RP; CIMMYT, Carretera Mexico-Veracruz Km 18, El Batan, Texcoco, Estado de México, Mexico.
  • Bariana H; Faculty of Science, School of Life and Environmental Sciences, The University of Sydney Plant Breeding Institute, 107 Cobbitty Road, Cobbitty, NSW, 2570, Australia. harbans.bariana@sydney.edu.au.
  • Lagudah E; CSIRO Agriculture and Food, P.O. Box 1700, Canberra, ACT, 2601, Australia. Evans.lagudah@csiro.au.
Theor Appl Genet ; 135(5): 1541-1550, 2022 May.
Article em En | MEDLINE | ID: mdl-35199199
ABSTRACT
KEY MESSAGE Adult plant stem rust resistance locus, QSrGH.cs-2AL, was identified in durum wheat Glossy Huguenot and mendelised as Sr63. Markers closely linked with Sr63 were developed. An F3 population from a Glossy Huguenot (GH)/Bansi cross used in a previous Australian study was advanced to F6 for molecular mapping of adult plant stem rust resistance. Maturity differences among F6 lines confounded assessments of stem rust response. GH was crossed with a stem rust susceptible F6 recombinant inbred line (RIL), GHB14 (M14), with similar maturity and an F67 population was developed through single seed descent method. F7 and F8 RILs were tested along with the parents at different locations. The F6 individual plants and both parents were genotyped using the 90 K single nucleotide polymorphism (SNP) wheat array. Stem rust resistance QTL on the long arms of chromosomes 1B (QSrGH.cs-1BL) and 2A (QSrGH.cs-2AL) were detected. QSrGH.cs-1BL and QSrGH.cs-2AL were both contributed by GH and explained 22% and 18% adult plant stem rust response variation, respectively, among GH/M14 RIL population. RILs carrying combinations of these QTL reduced more than 14% stem rust severity compared to those that possessed QSrGH.cs-1BL and QSrGH.cs-2AL individually. QSrGH.cs1BL was demonstrated to be the same as Sr58/Lr46/Yr29/Pm39 through marker genotyping. Lines lacking QSrGH.cs-1BL were used to Mendelise QSrGH.cs-2AL. Based on genomic locations of previously catalogued stem rust resistance genes and the QSrGH.cs-2AL map, it appeared to represent a new APR locus and was permanently named Sr63. SNP markers associated with Sr63 were converted to kompetetive allele-specific PCR (KASP) assays and were validated on a set of durum cultivars.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Basidiomycota / Triticum Tipo de estudo: Prognostic_studies País/Região como assunto: Oceania Idioma: En Revista: Theor Appl Genet Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Austrália
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Basidiomycota / Triticum Tipo de estudo: Prognostic_studies País/Região como assunto: Oceania Idioma: En Revista: Theor Appl Genet Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Austrália