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Genomic and Phenotypic Characterization of Clostridium botulinum Isolates from an Infant Botulism Case Suggests Adaptation Signatures to the Gut.
Douillard, François P; Derman, Yagmur; Woudstra, Cédric; Selby, Katja; Mäklin, Tommi; Dorner, Martin B; Saxén, Harri; Dorner, Brigitte G; Korkeala, Hannu; Lindström, Miia.
Afiliação
  • Douillard FP; Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinkigrid.7737.4, Helsinki, Finland.
  • Derman Y; Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinkigrid.7737.4, Helsinki, Finland.
  • Woudstra C; Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinkigrid.7737.4, Helsinki, Finland.
  • Selby K; Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinkigrid.7737.4, Helsinki, Finland.
  • Mäklin T; Helsinki Institute for Information Technology HIIT, Department of Mathematics and Statistics, University of Helsinkigrid.7737.4, Helsinki, Finland.
  • Dorner MB; Biological Toxins, Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany.
  • Saxén H; New Children's Hospital, Pediatric Research Center, University of Helsinkigrid.7737.4 and Helsinki University Hospital, Helsinki, Finland.
  • Dorner BG; Biological Toxins, Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany.
  • Korkeala H; Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinkigrid.7737.4, Helsinki, Finland.
  • Lindström M; Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinkigrid.7737.4, Helsinki, Finland.
mBio ; 13(3): e0238421, 2022 06 28.
Article em En | MEDLINE | ID: mdl-35499308
In early life, the immature human gut microbiota is prone to colonization by pathogens that are usually outcompeted by mature microbiota in the adult gut. Colonization and neurotoxin production by a vegetative Clostridium botulinum culture in the gut of an infant can lead to flaccid paralysis, resulting in a clinical outcome known as infant botulism, a potentially life-threatening condition. Beside host factors, little is known of the ecology, colonization, and adaptation of C. botulinum to the gut environment. In our previous report, an infant with intestinal botulism was shown to be colonized by neurotoxigenic C. botulinum culture for 7 months. In an effort to gain ecological and evolutionary insights into this unusually long gut colonization by C. botulinum, we analyzed and compared the genomes of C. botulinum isolates recovered from the infant feces during the course of intoxication and isolates from the infant household dust. A number of observed mutations and genomic alterations pinpointed at phenotypic traits that may have promoted colonization and adaptation to the gut environment and to the host. These traits include motility, quorum-sensing, sporulation, and carbohydrate metabolism. We provide novel perspectives and suggest a tentative model of the pathogenesis of C. botulinum in infant botulism. IMPORTANCE While the clinical aspects of infant botulism and the mode of action of BoNT have been thoroughly investigated, little is known on the pathogenesis and adaptive mechanisms of C. botulinum in the gut. Here, we provide for the first time a comprehensive view on the genomic dynamics and plasticity of C. botulinum over time in a case of infant botulism. The genomic and phenotypic analysis of C. botulinum isolates collected during the disease course offers an unprecedented view of C. botulinum ecology, evolution, and pathogenesis and may be instrumental in developing novel strategies for prevention and treatment of toxicoinfectious botulism.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Botulismo / Clostridium botulinum / Microbioma Gastrointestinal Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Humans / Infant Idioma: En Revista: MBio Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Finlândia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Botulismo / Clostridium botulinum / Microbioma Gastrointestinal Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Humans / Infant Idioma: En Revista: MBio Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Finlândia