Assay for heat-labile Escherichia coli enterotoxin using sandwich erythroimmunoassay.
Med Microbiol Immunol
; 176(2): 89-97, 1987.
Article
em En
| MEDLINE
| ID: mdl-3553884
ABSTRACT
We investigated the possibility of using a sheep erythrocyte-antibody conjugate as reagent in a sandwich erythroimmunoassay (SERIA) procedure to detect and titrate Escherichia coli heat-labile enterotoxin (LT) with the naked eye. In this assay, which is based on the immunological similarity between Vibrio cholerae toxin (CT) and LT, rabbit anti-CT IgG was used as immunosorbent, and sheep erythrocytes, sensitized with the rabbit anti-CT antibodies, were used as indicator. The sensitivity of the test was demonstrated by a comparative study using an enzyme-linked immunosorbent assay (ELISA). The results obtained by SERIA with 130 samples correlated well with those of a Vero cell assay and a GM1-ELISA. The test is easy, relatively cheap and as sensitive as other standard techniques; it is particularly suited for field laboratories, especially in tropical countries, and a large number of strains may be examined daily.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
/
Proteínas de Escherichia coli
/
Enterotoxinas
/
Escherichia coli
Limite:
Animals
Idioma:
En
Revista:
Med Microbiol Immunol
Ano de publicação:
1987
Tipo de documento:
Article