Genetic trade-offs and unexpected life history traits shape local adaptation in Trifolium repens.

ABSTRACT

Local adaptation has evolved in numerous taxa across the tree of life in response to divergent selection acting on populations that inhabit different environments (Briscoe Runquist et al., 2019; Hargreaves et al., 2019; Hereford, 2009). The genetic basis of local adaptation has intrigued researchers for decades. In their foundational reciprocal transplant study, Clausen and Hiesey (1960) evaluated the genetics of adaptation primarily using hybrid lines derived from crosses of low elevation and alpine Potentilla glandulosa (Rosaceae) ecotypes. Their work revealed that transgressive segregation can lead to a wider range of trait values than expressed by the parents, complex traits are often genetically correlated and evolve in tandem, and local adaptation is typically polygenic, that is, controlled by many loci of small effect (Clausen & Hiesey, 1960). Within the past 15 years, a burgeoning literature has investigated whether local adaptation evolves through genetic trade-offs, such that local alleles at a QTL (quantitative trait locus) or candidate gene have a fitness advantage in their home environment, but suffer a fitness cost when transplanted into a contrasting habitat type (Mitchell-Olds et al., 2007). Alternatively, local adaptation could arise through conditional neutrality, in which an allele native to one habitat type has elevated fitness in its home site relative to foreign alleles, but is not at a fitness disadvantage elsewhere. Conditional neutrality could maintain local adaptation if gene flow is spatially restricted (Hall et al., 2010). In a From the Cover article in this issue of Molecular Ecology, Wright et al. (2022) examined the genetic basis of local adaptation in white clover (Trifolium repens), discovering strong signatures of local adaptation, and revealing that both genetic trade-offs and conditional neutrality contribute to local adaptation. The most surprising and intriguing result to emerge from this study was that variation in a key antiherbivore defence did not appear to influence contemporary patterns of local adaptation. Rather, divergence in life history strategies was crucial, with early reproduction favoured in the southern garden and delayed reproduction and longer lifespans emerging in the north. These findings highlight the challenges of identifying the multivariate targets of divergent selection in locally-adapted systems, and reveal that not all traits that vary across populations contribute to adaptive differentiation. As studies continue to investigate local adaptation, experiments that manipulate environmental conditions and quantify the magnitude and direction of selection on traits will shed light on the processes that drive local adaptation.