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Exosomes as Carriers for Notch Molecules.
Marconi, Guya Diletta; Diomede, Francesca; Trubiani, Oriana; Porcheri, Cristina; Mitsiadis, Thimios A.
Afiliação
  • Marconi GD; Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
  • Diomede F; Orofacial Development and Regeneration, Institute of Oral Biology, University of Zurich, Zurich, Switzerland.
  • Trubiani O; Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
  • Porcheri C; Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
  • Mitsiadis TA; Orofacial Development and Regeneration, Institute of Oral Biology, University of Zurich, Zurich, Switzerland.
Methods Mol Biol ; 2472: 197-208, 2022.
Article em En | MEDLINE | ID: mdl-35674902
ABSTRACT
Exosomes are extracellular vesicles involved in cell-to-cell communication as well as extrusion of biological material. Using dental pulp stem cells culture as a model, we hereby describe a method for the packaging of Delta-like 4 (DLL4), a representative Notch ligand, into newly generated exosomes. We then provide methods of analysis to confirm the presence of Notch proteins and transcripts internalization and transport via exosomes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Exossomos / Vesículas Extracelulares Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Itália
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Exossomos / Vesículas Extracelulares Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Itália