Your browser doesn't support javascript.
loading
Sulfated glycan recognition by carbohydrate sulfatases of the human gut microbiota.
Luis, Ana S; Baslé, Arnaud; Byrne, Dominic P; Wright, Gareth S A; London, James A; Jin, Chunsheng; Karlsson, Niclas G; Hansson, Gunnar C; Eyers, Patrick A; Czjzek, Mirjam; Barbeyron, Tristan; Yates, Edwin A; Martens, Eric C; Cartmell, Alan.
Afiliação
  • Luis AS; Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA. ana.luis@medkem.gu.se.
  • Baslé A; Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Gothenburg, Sweden. ana.luis@medkem.gu.se.
  • Byrne DP; Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK.
  • Wright GSA; Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.
  • London JA; Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.
  • Jin C; Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.
  • Karlsson NG; Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Gothenburg, Sweden.
  • Hansson GC; Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Gothenburg, Sweden.
  • Eyers PA; Faculty of Health Sciences, Department of Life Sciences and Health, Pharmacy, Oslo Metropolitan University, Oslo, Norway.
  • Czjzek M; Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Gothenburg, Sweden.
  • Barbeyron T; Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.
  • Yates EA; Sorbonne Université, Univ Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS, Roscoff, France.
  • Martens EC; Sorbonne Université, Univ Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS, Roscoff, France.
  • Cartmell A; Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.
Nat Chem Biol ; 18(8): 841-849, 2022 08.
Article em En | MEDLINE | ID: mdl-35710619
Sulfated glycans are ubiquitous nutrient sources for microbial communities that have coevolved with eukaryotic hosts. Bacteria metabolize sulfated glycans by deploying carbohydrate sulfatases that remove sulfate esters. Despite the biological importance of sulfatases, the mechanisms underlying their ability to recognize their glycan substrate remain poorly understood. Here, we use structural biology to determine how sulfatases from the human gut microbiota recognize sulfated glycans. We reveal seven new carbohydrate sulfatase structures spanning four S1 sulfatase subfamilies. Structures of S1_16 and S1_46 represent novel structures of these subfamilies. Structures of S1_11 and S1_15 demonstrate how non-conserved regions of the protein drive specificity toward related but distinct glycan targets. Collectively, these data reveal that carbohydrate sulfatases are highly selective for the glycan component of their substrate. These data provide new approaches for probing sulfated glycan metabolism while revealing the roles carbohydrate sulfatases play in host glycan catabolism.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sulfatases / Microbioma Gastrointestinal Limite: Humans Idioma: En Revista: Nat Chem Biol Assunto da revista: BIOLOGIA / QUIMICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sulfatases / Microbioma Gastrointestinal Limite: Humans Idioma: En Revista: Nat Chem Biol Assunto da revista: BIOLOGIA / QUIMICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos